Document Detail

Farnesol and farnesal dehydrogenase(s) in corpora allata of the tobacco hornworm moth, Manduca sexta.
MedLine Citation:
PMID:  6366103     Owner:  NLM     Status:  MEDLINE    
The metabolism of [3H]farnesol was studied in cell-free preparations of corpora allata from the tobacco hornworm, Manduca sexta, to assess the role of this presumed biosynthetic precursor of juvenile hormone (JH) III. A reversed-phase ion-pair liquid chromatographic (RP-IPC) procedure was devised to separate farnesol from several potential intermediates in its presumed metabolism to JH III: farnesal, farnesoic acid, 10,11-epoxyfarnesoic acid, and methyl farnesoate. Following incubation of (2E,6E)-[1,5,9-3H]farnesol with homogenates of corpora allata from fifth instar larvae or adult female M. sexta, and analysis by RP-IPC, the major radiolabeled products corresponded to farnesoic acid, farnesal, and a polar product(s) presumably derived from the tritium on C-1 of farnesol. Inclusion of NAD+ in the incubations conducted with crude homogenates resulted in enhanced [3H]farnesol metabolism, decreased accumulation of [3H]farnesal, and increased levels of [3H]farnesoic acid. Substitution of NADP+ for NAD+ was ineffective, suggesting that farnesol and/or farnesal dehydrogenase were NAD+-dependent enzymes. Pellet fractions obtained by differential centrifugation of crude homogenates exhibited both farnesol and farnesal dehydrogenase activity but only the latter was clearly stimulated by addition of NAD+. The alcohol/aldehyde dehydrogenase(s) showed some substrate specificity for the 2E isomer; nerol and (2Z,6E)-farnesol were barely metabolized under conditions in which either geraniol or (2E,6E)-farnesol were rapidly oxidized. The identity of the [3H]farnesal zone obtained from RP-IPC was further established by normal-phase liquid chromatography and by gas-liquid chromatography-mass spectrometry.(ABSTRACT TRUNCATED AT 250 WORDS)
F C Baker; B Mauchamp; L W Tsai; D A Schooley
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of lipid research     Volume:  24     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1983 Dec 
Date Detail:
Created Date:  1984-04-11     Completed Date:  1984-04-11     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1586-94     Citation Subset:  IM    
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MeSH Terms
Alcohol Oxidoreductases / metabolism*
Aldehyde Oxidoreductases / metabolism*
Farnesol / metabolism*
Juvenile Hormones / biosynthesis
Larva / metabolism
Lepidoptera / metabolism*
Moths / metabolism*
Polyisoprenyl Phosphates / metabolism
Tritium / diagnostic use
Reg. No./Substance:
0/Juvenile Hormones; 0/Polyisoprenyl Phosphates; 0/Sesquiterpenes; 10028-17-8/Tritium; 13058-04-3/farnesyl pyrophosphate; 4602-84-0/Farnesol; EC 1.1.-/Alcohol Oxidoreductases; EC 1.1.1.-/farnesol dehydrogenase; EC 1.2.-/Aldehyde Oxidoreductases; EC 1.2.1.-/farnesal dehydrogenase

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