| Farnesol and farnesal dehydrogenase(s) in corpora allata of the tobacco hornworm moth, Manduca sexta. | |
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MedLine Citation:
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PMID: 6366103 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The metabolism of [3H]farnesol was studied in cell-free preparations of corpora allata from the tobacco hornworm, Manduca sexta, to assess the role of this presumed biosynthetic precursor of juvenile hormone (JH) III. A reversed-phase ion-pair liquid chromatographic (RP-IPC) procedure was devised to separate farnesol from several potential intermediates in its presumed metabolism to JH III: farnesal, farnesoic acid, 10,11-epoxyfarnesoic acid, and methyl farnesoate. Following incubation of (2E,6E)-[1,5,9-3H]farnesol with homogenates of corpora allata from fifth instar larvae or adult female M. sexta, and analysis by RP-IPC, the major radiolabeled products corresponded to farnesoic acid, farnesal, and a polar product(s) presumably derived from the tritium on C-1 of farnesol. Inclusion of NAD+ in the incubations conducted with crude homogenates resulted in enhanced [3H]farnesol metabolism, decreased accumulation of [3H]farnesal, and increased levels of [3H]farnesoic acid. Substitution of NADP+ for NAD+ was ineffective, suggesting that farnesol and/or farnesal dehydrogenase were NAD+-dependent enzymes. Pellet fractions obtained by differential centrifugation of crude homogenates exhibited both farnesol and farnesal dehydrogenase activity but only the latter was clearly stimulated by addition of NAD+. The alcohol/aldehyde dehydrogenase(s) showed some substrate specificity for the 2E isomer; nerol and (2Z,6E)-farnesol were barely metabolized under conditions in which either geraniol or (2E,6E)-farnesol were rapidly oxidized. The identity of the [3H]farnesal zone obtained from RP-IPC was further established by normal-phase liquid chromatography and by gas-liquid chromatography-mass spectrometry.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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F C Baker; B Mauchamp; L W Tsai; D A Schooley |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of lipid research Volume: 24 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 1983 Dec |
Date Detail:
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Created Date: 1984-04-11 Completed Date: 1984-04-11 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1586-94 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alcohol Oxidoreductases
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metabolism* Aldehyde Oxidoreductases / metabolism* Animals Farnesol / metabolism* Female Juvenile Hormones / biosynthesis Larva / metabolism Lepidoptera / metabolism* Moths / metabolism* Polyisoprenyl Phosphates / metabolism Sesquiterpenes Tritium / diagnostic use |
| Chemical | |
Reg. No./Substance:
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0/Juvenile Hormones; 0/Polyisoprenyl Phosphates; 0/Sesquiterpenes; 10028-17-8/Tritium; 13058-04-3/farnesyl pyrophosphate; 4602-84-0/Farnesol; EC 1.1.-/Alcohol Oxidoreductases; EC 1.1.1.-/farnesol dehydrogenase; EC 1.2.-/Aldehyde Oxidoreductases; EC 1.2.1.-/farnesal dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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