Document Detail

Far3 and five interacting proteins prevent premature recovery from pheromone arrest in the budding yeast Saccharomyces cerevisiae.
MedLine Citation:
PMID:  12588993     Owner:  NLM     Status:  MEDLINE    
In budding yeast, diffusible mating pheromones initiate a signaling pathway that culminates in several responses, including cell cycle arrest. Only a handful of genes required for the interface between pheromone response and the cell cycle have been identified, among them FAR1 and FAR3; of these, only FAR1 has been extensively characterized. In an effort to learn about the mechanism by which Far3 acts, we used the two-hybrid method to identify interacting proteins. We identified five previously uncharacterized open reading frames, dubbed FAR7, FAR8, FAR9, FAR10, and FAR11, that cause a far3-like pheromone arrest defect when disrupted. Using two-hybrid and coimmunoprecipitation analysis, we found that all six Far proteins interact with each other. Moreover, velocity sedimentation experiments suggest that Far3 and Far7 to Far11 form a complex. The phenotype of a sextuple far3far7-far11 mutant is no more severe than any single mutant. Thus, FAR3 and FAR7 to FAR11 all participate in the same pathway leading to G1 arrest. These mutants initially arrest in response to pheromone but resume budding after 10 h. Under these conditions, wild-type cells fail to resume budding even after several days whereas far1 mutant cells resume budding within 1 h. We conclude that the FAR3-dependent arrest pathway is functionally distinct from that which employs FAR1.
Hilary A Kemp; George F Sprague
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  23     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-02-17     Completed Date:  2003-04-04     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1750-63     Citation Subset:  IM    
Institute of Molecular Biology and Department of Biology, University of Oregon, Eugene, Oregon 97403-1229, USA.
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MeSH Terms
Amino Acid Sequence
Blotting, Western
Cell Cycle
Cell Cycle Proteins / metabolism*
Centrifugation, Density Gradient
Electrophoresis, Polyacrylamide Gel
G1 Phase
Models, Biological
Molecular Sequence Data
Pheromones / metabolism
Plasmids / metabolism
Precipitin Tests
Protein Structure, Tertiary
Saccharomyces cerevisiae / metabolism
Saccharomyces cerevisiae Proteins / metabolism*
Sequence Homology, Amino Acid
Signal Transduction
Time Factors
Two-Hybrid System Techniques
beta-Galactosidase / metabolism
Grant Support
Reg. No./Substance:
0/Cell Cycle Proteins; 0/FAR11 protein, S cerevisiae; 0/FAR3 protein, S cerevisiae; 0/FAR7 protein, S cerevisiae; 0/FAR9 protein, S cerevisiae; 0/Pheromones; 0/Saccharomyces cerevisiae Proteins; EC

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