Document Detail


Fanconi anemia lymphocytes: effect of DL-alpha-tocopherol (Vitamin E) on chromatid breaks and on G2 repair efficiency.
MedLine Citation:
PMID:  11104902     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The high frequency of chromosomal breaks in Fanconi anemia (FA) lymphocytes has been related to the increased oxidative damage shown by these cells. The effect of 100 microM DL-alpha-tocopherol (Vitamin E) on the level of chromosomal damage in mitosis was studied in lymphocytes from five FA patients and from age matched controls, both under basal conditions and when G2 repair was prevented by 2.5 mM caffeine (G2 unrepaired damage). In addition, the effect of this antioxidant on G2 duration and the efficiency of G2 repair was also evaluated in the sample. alpha-Tocopherol (AT) decreased the frequency of chromosomal damage (under basal and inhibited G2 repair conditions) and the duration of G2 in FA cells. This antioxidant protective effect, expressed as the decrease in chromatid breaks, was greater in FA cells (50.8%) than in controls (25%). The efficiency of the G2 repair process (G2 R rate) defined as the ratio between the percentage of chromatid breaks repaired in G2 and the duration of this cell cycle phase was lesser in FA cells (10.6) than in controls (22.6). AT treatment slightly increased this G2 R rate, both in FA cells and controls. These results suggest that an increased oxidative damage and a lower G2 repair rate may be simultaneously involved in the high frequency of chromatid damage detected in FA cells.
Authors:
J Pincheira; M Bravo; M J Santos; de la Torre C; J F López-Sáez
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Mutation research     Volume:  461     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-01-26     Completed Date:  2001-02-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  265-71     Citation Subset:  IM    
Affiliation:
Programa de Genética Humana, y Facultad de Medicina, Universidad de Chile, Casilla 70061, Santiago 7, Chile.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Child
Chromatids / drug effects*
Chromosome Aberrations
DNA / drug effects,  metabolism
DNA Repair
Fanconi Anemia / pathology*
Female
G2 Phase / drug effects,  genetics
Humans
Lymphocytes / drug effects*,  physiology
Male
Vitamin E / pharmacology*
Chemical
Reg. No./Substance:
1406-18-4/Vitamin E; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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