| Fanconi anemia lymphocytes: effect of DL-alpha-tocopherol (Vitamin E) on chromatid breaks and on G2 repair efficiency. | |
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MedLine Citation:
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PMID: 11104902 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The high frequency of chromosomal breaks in Fanconi anemia (FA) lymphocytes has been related to the increased oxidative damage shown by these cells. The effect of 100 microM DL-alpha-tocopherol (Vitamin E) on the level of chromosomal damage in mitosis was studied in lymphocytes from five FA patients and from age matched controls, both under basal conditions and when G2 repair was prevented by 2.5 mM caffeine (G2 unrepaired damage). In addition, the effect of this antioxidant on G2 duration and the efficiency of G2 repair was also evaluated in the sample. alpha-Tocopherol (AT) decreased the frequency of chromosomal damage (under basal and inhibited G2 repair conditions) and the duration of G2 in FA cells. This antioxidant protective effect, expressed as the decrease in chromatid breaks, was greater in FA cells (50.8%) than in controls (25%). The efficiency of the G2 repair process (G2 R rate) defined as the ratio between the percentage of chromatid breaks repaired in G2 and the duration of this cell cycle phase was lesser in FA cells (10.6) than in controls (22.6). AT treatment slightly increased this G2 R rate, both in FA cells and controls. These results suggest that an increased oxidative damage and a lower G2 repair rate may be simultaneously involved in the high frequency of chromatid damage detected in FA cells. |
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Authors:
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J Pincheira; M Bravo; M J Santos; de la Torre C; J F López-Sáez |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Mutation research Volume: 461 ISSN: 0027-5107 ISO Abbreviation: Mutat. Res. Publication Date: 2001 Jan |
Date Detail:
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Created Date: 2001-01-26 Completed Date: 2001-02-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0400763 Medline TA: Mutat Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 265-71 Citation Subset: IM |
Affiliation:
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Programa de Genética Humana, y Facultad de Medicina, Universidad de Chile, Casilla 70061, Santiago 7, Chile. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Child Chromatids / drug effects* Chromosome Aberrations DNA / drug effects, metabolism DNA Repair Fanconi Anemia / pathology* Female G2 Phase / drug effects, genetics Humans Lymphocytes / drug effects*, physiology Male Vitamin E / pharmacology* |
| Chemical | |
Reg. No./Substance:
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1406-18-4/Vitamin E; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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