|False positive rate of an arrayCGH platform for single-cell preimplantation genetic screening and subsequent clinical application on day-3.|
|PMID: 23254309 Owner: NLM Status: MEDLINE|
|In this work, false positive rate of an arrayCGH platform for its use in day-3 single-blastomere analysis was calculated. For this purpose, 38 embryos diagnosed as abnormal on day-3 by FISH were re-biopsied on day-4. Single-cell day-4 arrayCGH diagnosis was then performed. A successful amplification was obtained in 97.4 % (37/38) of the day-4 cells analysed by arrayCGH. Day-3 FISH and day-4 arrayCGH diagnosis were concordant in 35/37 cases. The two discordant embryos were spread and all the cells from each embryo were re-analysed by FISH on day 5. The same error rate (2.7 %) for day-3 FISH and day-4 arrayCGH was obtained when comparing day-5 FISH re-analysis. After this pre-clinical phase, the platform was used for day-3 arrayCGH clinical application in 320 patients (1,760 embryos). Day-3 amplification rate was 98.6 %. An optimal reproductive outcome was obtained when applying arrayCGH to a clinical program: clinical pregnancy rate per cycle of 38.4 % and 60.3 % per transference were obtained, with an implantation rate of 53.5 %. Overall miscarriage rate was 10.6 %. Additionally, day-5 FISH re-analysis was performed in 42 of the embryos from the clinical phase, obtaining a concordance rate of 97.6 % with day-3 arrayCGH.|
|Pere Mir; Lorena Rodrigo; Amparo Mercader; Pilar Buendía; Emilia Mateu; Miguel Milán-Sánchez; Vanessa Peinado; Antonio Pellicer; Jose Remohí; Carlos Simón; Carmen Rubio|
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|Type: Journal Article Date: 2012-12-20|
|Title: Journal of assisted reproduction and genetics Volume: 30 ISSN: 1573-7330 ISO Abbreviation: J. Assist. Reprod. Genet. Publication Date: 2013 Jan|
|Created Date: 2013-01-24 Completed Date: 2013-07-22 Revised Date: 2014-01-09|
Medline Journal Info:
|Nlm Unique ID: 9206495 Medline TA: J Assist Reprod Genet Country: Netherlands|
|Languages: eng Pagination: 143-9 Citation Subset: IM|
|APA/MLA Format Download EndNote Download BibTex|
Blastocyst / cytology*
Blastomeres / cytology*
Chromosomes, Human / genetics
Comparative Genomic Hybridization / methods*
False Positive Reactions
Genetic Testing / methods*
In Situ Hybridization, Fluorescence
Reproducibility of Results
Sensitivity and Specificity
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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