Document Detail


False positive rate of an arrayCGH platform for single-cell preimplantation genetic screening and subsequent clinical application on day-3.
MedLine Citation:
PMID:  23254309     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this work, false positive rate of an arrayCGH platform for its use in day-3 single-blastomere analysis was calculated. For this purpose, 38 embryos diagnosed as abnormal on day-3 by FISH were re-biopsied on day-4. Single-cell day-4 arrayCGH diagnosis was then performed. A successful amplification was obtained in 97.4 % (37/38) of the day-4 cells analysed by arrayCGH. Day-3 FISH and day-4 arrayCGH diagnosis were concordant in 35/37 cases. The two discordant embryos were spread and all the cells from each embryo were re-analysed by FISH on day 5. The same error rate (2.7 %) for day-3 FISH and day-4 arrayCGH was obtained when comparing day-5 FISH re-analysis. After this pre-clinical phase, the platform was used for day-3 arrayCGH clinical application in 320 patients (1,760 embryos). Day-3 amplification rate was 98.6 %. An optimal reproductive outcome was obtained when applying arrayCGH to a clinical program: clinical pregnancy rate per cycle of 38.4 % and 60.3 % per transference were obtained, with an implantation rate of 53.5 %. Overall miscarriage rate was 10.6 %. Additionally, day-5 FISH re-analysis was performed in 42 of the embryos from the clinical phase, obtaining a concordance rate of 97.6 % with day-3 arrayCGH.
Authors:
Pere Mir; Lorena Rodrigo; Amparo Mercader; Pilar Buendía; Emilia Mateu; Miguel Milán-Sánchez; Vanessa Peinado; Antonio Pellicer; Jose Remohí; Carlos Simón; Carmen Rubio
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Publication Detail:
Type:  Journal Article     Date:  2012-12-20
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  30     ISSN:  1573-7330     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-24     Completed Date:  2013-07-22     Revised Date:  2014-01-09    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  143-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Aneuploidy
Biopsy
Blastocyst / cytology*
Blastomeres / cytology*
Chromosomes, Human / genetics
Comparative Genomic Hybridization / methods*
Cryopreservation
Embryo Implantation
Embryo Transfer
False Positive Reactions
Female
Genetic Testing / methods*
Humans
In Situ Hybridization, Fluorescence
Pregnancy
Pregnancy Rate
Prospective Studies
Reproducibility of Results
Sensitivity and Specificity
Time Factors
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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