Document Detail

Failure to detect Chlamydia pneumoniae DNA in cerebral aneurysmal sac tissue with two different polymerase chain reaction methods.
MedLine Citation:
PMID:  12754346     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: Chlamydia pneumoniae (C pneumoniae) is a common cause of a usually mild, community acquired pneumonia. This organism, however, can spread from the respiratory tract into other parts of the body and has been detected in up to 70% of atheromatous lesions in blood vessels. Although the exact mechanism of the C Pneumoniae contribution to the pathogenesis of atherosclerosis remains unknown, prophylactic antibiotic trials are planned for people at high risk for coronary disease. METHOD: In this study the authors aimed to investigate C pneumoniae DNA content in the cerebral aneurysmal sac tissue with the aid of polymerase chain reaction (PCR) method. C pneumoniae DNA was searched in 15 surgically clipped and removed aneurysmal sac tissue and in two tumour (an ependymoma of the fourth ventricle and a craniofaringoma) samples by touchdown enzyme time release PCR (TETR PCR) targeting 16S rRNA gene and by nested PCR targeting ompA gene. RESULTS: Both PCR methods were sensitive to detect in C pneumoniae 4x10(-2) genomes. C pneumoniae DNA was not detected in any of the 17 sample tissues of these patients. CONCLUSION: The contribution of C pneumoniae in the development of intracranial aneurysms cannot be excluded despite the results of this study. Further studies on the possible role of C pneumoniae or any other micro-organisms in the pathogenesis of aneurysms should be performed.
S Cagli; N Oktar; T Dalbasti; S Erensoy; N Ozdamar; S Göksel; A Sayiner; A Bilgiç
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Journal of neurology, neurosurgery, and psychiatry     Volume:  74     ISSN:  0022-3050     ISO Abbreviation:  J. Neurol. Neurosurg. Psychiatr.     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-05-19     Completed Date:  2003-07-08     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985191R     Medline TA:  J Neurol Neurosurg Psychiatry     Country:  England    
Other Details:
Languages:  eng     Pagination:  756-9     Citation Subset:  IM    
Department of Neurosurgery, Ege University School of Medicine, Izmir, Turkey.
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MeSH Terms
Aneurysm, Infected / genetics*,  microbiology*
Chlamydia Infections / genetics*,  microbiology*
Chlamydophila pneumoniae / genetics*,  isolation & purification*
DNA Primers / genetics
DNA, Bacterial / analysis
Intracranial Aneurysm / microbiology*
Middle Aged
Polymerase Chain Reaction / methods*
Reg. No./Substance:
0/DNA Primers; 0/DNA, Bacterial
Erratum In:
J Neurol Neurosurg Psychiatry. 2003 Aug;74(8):1165

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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