| Failure in S6 protein phosphorylation by serum stimulation of senescent human diploid fibroblasts, TIG-1. | |
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MedLine Citation:
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PMID: 3821186 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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When quiescent young or senescent human diploid cells, TIG-1, were metabolically labeled with 32Pi and stimulated with 10% fetal bovine serum, the phosphorylation of ribosomal S6 protein was enhanced in young cells but not in senescent cells while that of some other proteins were increased in both cells. Inability to stimulate the phosphorylation of S6 protein in senescent cells after serum addition may be the primary cause of the failure of enhancement in protein synthesis followed by the block of prereplicative events dependent on protein synthesis and thus of the failure of cells to enter S phase. However, when the cell-free preparation from serum-stimulated senescent cells was incubated with [gamma-32P]ATP, S6-kinase activity was stimulated and S6 in ribosomal fraction was susceptible to phosphorylation as observed in young cells. Differences in S6 phosphorylation of senescent cells between in vivo and in vitro was discussed. |
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Authors:
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F Kihara; J Ninomiya-Tsuji; S Ishibashi; T Ide |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Mechanisms of ageing and development Volume: 37 ISSN: 0047-6374 ISO Abbreviation: Mech. Ageing Dev. Publication Date: 1986 Nov |
Date Detail:
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Created Date: 1987-04-15 Completed Date: 1987-04-15 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0347227 Medline TA: Mech Ageing Dev Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 27-40 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Blood* Cell Survival Electrophoresis, Polyacrylamide Gel Fibroblasts / cytology*, metabolism Humans Molecular Weight Phosphorylation Ribosomal Protein S6 Ribosomal Proteins / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Ribosomal Protein S6; 0/Ribosomal Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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