| Failure of endothelin-1 to activate store-operated Ca2+ channels by lack of mobilization from intracellular Ca2+ stores in cultured bovine adrenal chromaffin cells. | |
| | |
MedLine Citation:
|
PMID: 11485037 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We have recently shown that in addition to L-type voltage-operated Ca2+ channel (VOC), endothelin-1 (ET-1) stimulation opens two types of Ca2+-permeable nonselective cation channels [designated nonselective cation channel-1 (NSCC-1) and NSCC-2]. However, in this Ca2+ entry, the involvement of store-operated Ca2+ channel (SOCC), which is suggested to exist in chromaffin cells, was unclear. Those NSCCs as well as SOCC can be pharmacologically discriminated using Ca2+ channel blockers such as SK&F 96365 and LOE 908. To clarify whether SOCC should actually exist and play a role in Ca2+ entry in chromaffin cells stimulated with ET-1, we examined the effects of removal of extracellular Ca2+, thapsigargin (TG, an inhibitor of endoplasmic reticulum Ca2+-ATPase), LOE 908 and SK&F 96365 on cytosolic free Ca2+ concentrations ([Ca2+]i) in cultured bovine adrenal chromaffin cells. After the cells were exposed to Ca2+-free medium followed by exposure to TG to deplete Ca2+ from the intracellular Ca2+ store, restoration of extracellular Ca2+ caused a gradual increase in [Ca2+]i (to about 200% of control). The increase was unaffected by LOE 908, but completely abolished by SK&F 96365. In the Ca2+-free medium, no increase in [Ca2+]i by ET-1 was observed, but the subsequent restoration of extracellular Ca2+ induced a rapid increase in [Ca2+]i (to the same level of [Ca2+]i as that evoked by ET-1 in the normal medium (1.0 mM Ca2+)). Since SK&F 96365 is also a blocker of SOCC, these results indicate that in bovine adrenal chromaffin cells, Ca2+ entry through SOCC (Ca2+ influx through the capacitative Ca2+ entry system) occurs but is comparably weak, and that it virtually does not work on the stimulation of ET-1. |
| | |
Authors:
|
K Lee; H Morita; Y Iwamuro; X F Zhang; Y Okamoto; S Miwa |
Related Documents
:
|
9595507 - Endothelin-1 inhibits pacemaker currents in rabbit sa node cells. 10619177 - Pharmacological characterization of receptor-mediated ca2+ entry in endothelin-1-induce... 1398887 - Phosphoramidon-sensitive effects of big endothelins in the perfused rabbit kidney. 1293317 - The effects of ca(2+)-free perfusion and the calcium paradox on [125i] endothelin-1 bin... 2870197 - Effect of selective kainate lesions on the release of glutamate and aspartate from chic... 9153247 - Beta subunit heterogeneity in neuronal l-type ca2+ channels. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Naunyn-Schmiedeberg's archives of pharmacology Volume: 364 ISSN: 0028-1298 ISO Abbreviation: Naunyn Schmiedebergs Arch. Pharmacol. Publication Date: 2001 Jul |
Date Detail:
|
Created Date: 2001-08-03 Completed Date: 2002-03-28 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0326264 Medline TA: Naunyn Schmiedebergs Arch Pharmacol Country: Germany |
Other Details:
|
Languages: eng Pagination: 42-6 Citation Subset: IM |
Affiliation:
|
Department of Physiology, Gifu University School of Medicine, Japan. kl@cc.gifu-u.ac.jp |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acetamides
/
pharmacology Animals Calcium / metabolism* Calcium Channel Blockers / pharmacology Calcium Channels, L-Type / metabolism* Cattle Cells, Cultured Chromaffin Cells / metabolism* Endothelin-1 / physiology* Enzyme Inhibitors / pharmacology Imidazoles / pharmacology* Ion Channels / drug effects Isoquinolines / pharmacology Thapsigargin / pharmacology |
| Chemical | |
Reg. No./Substance:
|
0/Acetamides; 0/Calcium Channel Blockers; 0/Calcium Channels, L-Type; 0/Endothelin-1; 0/Enzyme Inhibitors; 0/Imidazoles; 0/Ion Channels; 0/Isoquinolines; 130495-35-1/1-(2-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenylethyl)-1H-imidazole; 149759-26-2/LOE 908; 67526-95-8/Thapsigargin; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effects of the cardioselective KATP channel blocker HMR 1098 on cardiac function in isolated perfuse...
Next Document: Glucose-induced insulin secretion is potentiated by a new imidazoline compound.