| Faecal elastase-1: lyophilization of stool samples prevents false low results in diarrhoea. | |
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MedLine Citation:
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PMID: 11444478 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: In patients with diarrhoea, faecal elastase-1 is used to detect exocrine pancreatic insufficiency. Diarrhoea is defined as >85% stool water content. METHODS: We analysed elastase-1 in 519 stool samples from 310 patients unprocessed as well as after lyophilization in a standard laboratory lyophilizator. Stool water content was calculated by weight difference before and after lyophilization. RESULTS: 151 stool samples were classified as 'diarrhoea' (mean stool water content 88.8%); all others had a mean water content of 75.4%. In the 'diarrhoea' samples, elastase-1 levels were considerably higher after lyophilization--with a calculated normal water content of 75% (606 +/- 359 microg/g, mean +/- s)--compared to measurement when unprocessed (279 +/- 151 microg/g; P < 0.0001). In 16 of the 151 cases (11%), the abnormally low elastase-1 level below 200 microg/g found in unprocessed stools proved normal (>200 microg/g) after lyophilization and correction for stool water content. CONCLUSION: Diarrhoea can result in falsely decreased elastase-1 levels in a number of patients with non-pancreatogenic diarrhoea. Lyophilization is a simple measure by which to determine elastase-1 independently of stool water content. Lyophilization of stool samples can therefore help to prevent wrong positive elastase-1 test results. |
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Authors:
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B Fischer; S Hoh; M Wehler; E G Hahn; H T Schneider |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Validation Studies |
Journal Detail:
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Title: Scandinavian journal of gastroenterology Volume: 36 ISSN: 0036-5521 ISO Abbreviation: Scand. J. Gastroenterol. Publication Date: 2001 Jul |
Date Detail:
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Created Date: 2001-07-10 Completed Date: 2001-12-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0060105 Medline TA: Scand J Gastroenterol Country: Norway |
Other Details:
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Languages: eng Pagination: 771-4 Citation Subset: IM |
Affiliation:
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Dept. of Medicine 1, University of Erlangen-Nürnberg, Erlangen, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Aged Aged, 80 and over Case-Control Studies Child Child, Preschool Diarrhea / etiology* Exocrine Pancreatic Insufficiency / complications*, diagnosis*, enzymology False Positive Reactions Feces / chemistry*, enzymology* Female Freeze Drying / methods*, standards* Humans Infant Male Middle Aged Pancreatic Elastase / analysis* Pancreatic Function Tests / methods*, standards* Sensitivity and Specificity Water / analysis |
| Chemical | |
Reg. No./Substance:
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7732-18-5/Water; EC 3.4.21.36/Pancreatic Elastase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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