Document Detail

Factors underlying the cell growth-related bystander responses to alpha particles.
MedLine Citation:
PMID:  10728689     Owner:  NLM     Status:  MEDLINE    
Increases in cell proliferation are widely viewed as being of importance in carcinogenesis. We report that exposure of normal human lung fibroblasts to a low dose of alpha particles like those emitted by radon/radon progeny stimulates their proliferation in vitro, and this response also occurs when unirradiated cells are treated with supernatants from alpha-irradiated cells. We attribute the promitogenic response to superoxide dismutase- and catalase-inhibitable a particle-induced increases in the concentrations of transforming growth factor beta1 (TGF-beta1) in cell supernatants. TGF-beta1 at concentrations commensurate with those in the supernatants capably induces increases in intracellular reactive oxygen species (ROS) in unirradiated cells. Furthermore, the addition of supernatants from alpha-irradiated cells to unirradiated cells decreases cellular levels of TP53 and CDKN1A and increases CDC2 and proliferating cell nuclear antigen in the latter. Like the increased intracellular ROS bystander effect, this "decreased TP53/CDKN1A response" can be mimicked in otherwise untreated cells by the addition of low concentrations of TGF-beta1. Our results indicate that alpha particle-associated increases in cell growth correlate with intracellular increases in ROS along with decreases in TP53 and CDKN1A, and that these cellular responses are mechanistically coupled. As well, the proliferating cell nuclear antigen and CDC2 increases that occur along with the decreased TP53/CDKN1A bystander effect also would expectedly favor enhanced cell growth. Such processes may account for cell hyperplastic responses in the conducting airways of the lower respiratory track that occur after inhalation exposure to radon/ radon progeny, as well as, perhaps, other ROS-associated environmental stresses.
R Iyer; B E Lehnert; R Svensson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer research     Volume:  60     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  2000 Mar 
Date Detail:
Created Date:  2000-04-11     Completed Date:  2000-04-11     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1290-8     Citation Subset:  IM    
Bioscience Division, Los Alamos National Laboratory, New Mexico 87545, USA.
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MeSH Terms
Alpha Particles*
Cell Division / radiation effects
Cells, Cultured
Cyclin-Dependent Kinase Inhibitor p21
Cyclins / metabolism
Dose-Response Relationship, Radiation
Fibroblasts / cytology*,  metabolism,  radiation effects*
Lung / cytology
Transforming Growth Factor beta / metabolism
Tumor Suppressor Protein p53 / metabolism
Grant Support
Reg. No./Substance:
0/CDKN1A protein, human; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Cyclins; 0/Transforming Growth Factor beta; 0/Tumor Suppressor Protein p53
Erratum In:
Cancer Res. 2003 Mar 15;63(6):1439

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