Document Detail

Factors mediating lipofection potency of a series of cationic phosphonolipids in human cell lines.
MedLine Citation:
PMID:  16730412     Owner:  NLM     Status:  MEDLINE    
A series of cationic liposomes known as cationic phosphonolipids (CPs) were evaluated as vehicles for in vitro gene transfer in K562 erythroleukemia cells and 5637 epithelial carcinoma cells. For each CP and target cell type examined, detailed analyses were performed to determine optimal transfection conditions (lipid/ DNA (+/-) charge ratio, amount of complexed episomal DNA, liposomal and lipoplex size, complexation medium and duration of complex-cell exposure time). Lipofection conditions were determined to be both cell- and lipid-type specific. Complexation medium critically affected transfection competence. The initial size of the liposome was not always predictive of lipofection potency. The lipid chemical composition had a strong impact upon lipofection efficiency; DOPE inclusion in the liposome formulations was found to affect the levels of transgene expression in a cell-dependent way. Notably, effective transgene expression was characterized by prominent plasmid nuclear incorporation. Human A gamma- and epsilon-globin transgene nuclear incorporation and expression in 5637 cells post GLB.391-mediated lipofection lends credence to its use as a vehicle of therapeutic transgene delivery.
Daphne Koumbi; Jean-Claude Clement; Zili Sideratou; Jean-Jacques Yaouanc; Dimitris Loukopoulos; Panagoula Kollia
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-04-19
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1760     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-07-03     Completed Date:  2006-08-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1151-9     Citation Subset:  IM    
First Department of Medicine, University of Athens, School of Medicine, Athens, Greece.
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MeSH Terms
Cell Line, Tumor
Nucleic Acid Hybridization
Phospholipids / chemistry*
Transfection / methods*
Reg. No./Substance:
0/Cations; 0/Phospholipids; 0/phosphonolipids

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