| Factors mediating lipofection potency of a series of cationic phosphonolipids in human cell lines. | |
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MedLine Citation:
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PMID: 16730412 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A series of cationic liposomes known as cationic phosphonolipids (CPs) were evaluated as vehicles for in vitro gene transfer in K562 erythroleukemia cells and 5637 epithelial carcinoma cells. For each CP and target cell type examined, detailed analyses were performed to determine optimal transfection conditions (lipid/ DNA (+/-) charge ratio, amount of complexed episomal DNA, liposomal and lipoplex size, complexation medium and duration of complex-cell exposure time). Lipofection conditions were determined to be both cell- and lipid-type specific. Complexation medium critically affected transfection competence. The initial size of the liposome was not always predictive of lipofection potency. The lipid chemical composition had a strong impact upon lipofection efficiency; DOPE inclusion in the liposome formulations was found to affect the levels of transgene expression in a cell-dependent way. Notably, effective transgene expression was characterized by prominent plasmid nuclear incorporation. Human A gamma- and epsilon-globin transgene nuclear incorporation and expression in 5637 cells post GLB.391-mediated lipofection lends credence to its use as a vehicle of therapeutic transgene delivery. |
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Authors:
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Daphne Koumbi; Jean-Claude Clement; Zili Sideratou; Jean-Jacques Yaouanc; Dimitris Loukopoulos; Panagoula Kollia |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-04-19 |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1760 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2006 Aug |
Date Detail:
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Created Date: 2006-07-03 Completed Date: 2006-08-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1151-9 Citation Subset: IM |
Affiliation:
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First Department of Medicine, University of Athens, School of Medicine, Athens, Greece. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cations Cell Line, Tumor Humans Kinetics Nucleic Acid Hybridization Phospholipids / chemistry* Plasmids Transfection / methods* |
| Chemical | |
Reg. No./Substance:
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0/Cations; 0/Phospholipids; 0/phosphonolipids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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