Document Detail

Factors involved in differential Giemsa-staining of sister chromatids.
MedLine Citation:
PMID:  77756     Owner:  NLM     Status:  MEDLINE    
Microspectrophotometric evaluation of differentially stained sister chromatids made it possible to analyse precisely the factors involved in the Giemsa methods. The concentration of Hoechst 33258, pH of the mounting medium temperature during UV-exposure and the quality (wavelength)of UV-light influenced the differential staining. Exposure of blacklight of 10(-5) M Hoechst 33528-stained brdU-labeled chromosome specimens mounted in McIlvaine buffer (pH 8.0) at 50 degrees C reproducibly allowed differential staining of sister chromatids within 15 min. On the other hand, Korenberg-Freedlender's method using no Hoechst 33258 was also UV-light-dependent. Thus, photolysis of BrdU-substituted DNA was considered the basic mechanism of the Giemsa methods where the photosensitive Hoechst 33258 played a role as a sensitizer.
K Goto; S Maeda; Y Kano; T Sugiyama
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chromosoma     Volume:  66     ISSN:  0009-5915     ISO Abbreviation:  Chromosoma     Publication Date:  1978 May 
Date Detail:
Created Date:  1978-08-28     Completed Date:  1978-08-28     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  2985138R     Medline TA:  Chromosoma     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  351-9     Citation Subset:  IM    
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MeSH Terms
Azure Stains
Staining and Labeling / methods*
Ultraviolet Rays
Reg. No./Substance:
0/Azure Stains

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