Document Detail


Factors interfering with the cell morphology in smears.
MedLine Citation:
PMID:  504967     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Factors interfering with the cell morphology in smears were studied. The following artifacts were observed: Lysis of cells with release of granules, cytoplasmic and nuclear proteins; fern-like structures due to detachment of protein from glass; rupture of the surface of smears; crystal formations; leakage from cells interfering with the surrounding medium; cell shrinkage, vacuolization; and aggregation of cells caused by gammaglobulins. The concentration and type of plasma protein in the medium greatly influenced the type of artifacts. The technique by which the least artifacts occurred, was cytocentrifugation of cells suspended in a cell protecting medium.
Authors:
I Talstad; J S Gundersen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Scandinavian journal of haematology     Volume:  23     ISSN:  0036-553X     ISO Abbreviation:  Scand J Haematol     Publication Date:  1979 Sep 
Date Detail:
Created Date:  1980-01-28     Completed Date:  1980-01-28     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0404507     Medline TA:  Scand J Haematol     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  197-203     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Blood Cells / cytology*
Cell Aggregation
Cell Nucleus
Crystallization
Cytoplasm
Humans
Neutrophils / cytology
Vacuoles

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