| Factors influencing the promotion of transformation in chemically-initiated C3H/10T1/2 Cl 8 mouse embryo fibroblasts. | |
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MedLine Citation:
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PMID: 6861275 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Treatment of low density asynchronous cultures of C3H/10T1/2 Cl 8 mouse embryo fibroblasts with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) initiates the process of transformation and produces significant numbers of transformed foci only when treated cultures are subsequently exposed to the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). Cell culture variables which influence the outcome of this initiation and promotion system were studied. A TPA concentration of 0.25 micrograms/ml was found to be optimal for the promotion of focus production and the presence of TPA was required both during logarithmic growth and throughout confluence. The lot of fetal calf serum used to cultivate the cells also played a determining role in focus production. Of nine serum lots purchased from four different suppliers, only two were suited for initiation and promotion studies with MNNG and TPA. In contrast, seven of these lots were adequate for transformation studies with 3-methylcholanthrene. Factors which adversely influenced focus production included the use of fungizone or the use of high passage stock cultures. These studies demonstrate that cell culture variables which influence promotion in these cells can be controlled and that this system can be successfully used in studies of the cellular mechanism of in vitro promotion and for the detection of genotoxic substances. |
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Authors:
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J H Frazelle; D J Abernethy; C J Boreiko |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Carcinogenesis Volume: 4 ISSN: 0143-3334 ISO Abbreviation: Carcinogenesis Publication Date: 1983 |
Date Detail:
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Created Date: 1983-08-11 Completed Date: 1983-08-11 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8008055 Medline TA: Carcinogenesis Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 709-15 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Transformation, Neoplastic / drug effects* Cells, Cultured Female Fibroblasts / drug effects Methylnitronitrosoguanidine / pharmacology* Mice Mice, Inbred C3H Phorbols / pharmacology* Pregnancy Tetradecanoylphorbol Acetate / pharmacology* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Phorbols; 16561-29-8/Tetradecanoylphorbol Acetate; 70-25-7/Methylnitronitrosoguanidine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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