| Factors influencing the performance of an interferon-γ assay for the diagnosis of tuberculosis in goats. | |
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MedLine Citation:
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PMID: 21050783 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The interferon-gamma (IFN-γ) assay is an effective tool for the diagnosis of tuberculosis (Tb) in goats. The objectives of this study were to evaluate factors that might affect assay performance: (1) the phenol concentration of the purified protein derivative (PPD, tuberculin) used; (2) dialysis of PPD; and (3) delaying antigenic stimulation of blood samples for 8, 16 and 24h after collection. The assay was performed in duplicate with two cut-off points. Dialysis of PPD reduced test sensitivity, whereas the concentration of phenol did not significantly affect test outcome. Delaying antigenic stimulation of samples >8h resulted in a reduction in test sensitivity, compromising the capacity of the assay to detect infected animals. Performing the assay in duplicate was unnecessary, which has implications for reducing assay costs. These findings will facilitate the effective application of the IFN-γ assay as an ancillary test in Tb eradication programmes in goats. |
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Authors:
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J Bezos; J Alvarez; L de Juan; B Romero; S Rodríguez; E Castellanos; J L Saéz-Llorente; A Mateos; L Domínguez; A Aranaz |
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Publication Detail:
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Type: Journal Article Date: 2010-11-02 |
Journal Detail:
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Title: Veterinary journal (London, England : 1997) Volume: 190 ISSN: 1532-2971 ISO Abbreviation: Vet. J. Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-10-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9706281 Medline TA: Vet J Country: England |
Other Details:
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Languages: eng Pagination: 131-5 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Centro VISAVET, Universidad Complutense de Madrid, 28040 Madrid, Spain; Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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