Document Detail

Factors influencing the oxygen consumption rate of aortic valve interstitial cells: application to tissue engineering.
MedLine Citation:
PMID:  19719392     Owner:  NLM     Status:  MEDLINE    
This study investigated the factors influencing the oxygen metabolism of aortic valve interstitial cells (VICs). Porcine VICs in cell suspension at different passages, and adhered to coverslips at different confluencies, as well as fresh porcine valve leaflets, were incubated in an oxygen respiration chamber at 37 degrees C in Dulbecco's modified Eagle's medium. The consumption rates at different oxygen concentrations were evaluated based on the Michaelis-Menten equation, and the corresponding maximum consumption rate (V(max)) and the Michaelis-Menten equation constant K(m) were determined. In all cases, the oxygen consumption rate was relatively constant until the concentration dropped to 5% (v/v). The metabolic activity of VICs in terms of oxygen consumption was dependent upon their in vitro passage number and proliferation status. These findings will provide valuable input to the selection of VICs with respect to their age and proliferation status for tissue engineering applications, as well as important input parameters for developing computational models of oxygen transport and optimization of the bioreactor conditions for heart valve tissue engineering.
Ling Wang; Stacy-Paul Wilshaw; Sotirios Korossis; John Fisher; Zhongmin Jin; Eileen Ingham
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Tissue engineering. Part C, Methods     Volume:  15     ISSN:  1937-3392     ISO Abbreviation:  -     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-09-01     Completed Date:  2009-11-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101466663     Medline TA:  Tissue Eng Part C Methods     Country:  United States    
Other Details:
Languages:  eng     Pagination:  355-63     Citation Subset:  IM    
Institute of Medical and Biological Engineering, University of Leeds, Leeds LS2 9JT, United Kingdom .
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MeSH Terms
Aortic Valve / cytology*,  physiology*
Cells, Cultured
Computer Simulation
Models, Cardiovascular*
Oxygen / metabolism*
Oxygen Consumption / physiology*
Tissue Engineering / methods*
Reg. No./Substance:

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