| Factors influencing in vitro respiratory burst assays with head kidney leucocytes from rainbow trout, Oncorhynchus mykiss (Walbaum). | |
| | |
MedLine Citation:
|
PMID: 20384908 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Abstract Head kidney leucocytes are central elements in a number of in vivo and in vitro assays elucidating innate and adaptive immune mechanisms in teleosts following stimulation with various antigens. These systems are sensitive to several factors affecting the outcome of the assays. The present work describes the importance of temperature, cell concentration, exposure time and immune-modulatory molecules on the respiratory burst activity (RBA) of rainbow trout head kidney leucocytes in vitro. Some variation in RBA was observed among individual fish. However, use of cells pooled from four individuals produced satisfactory results following exposure to phorbol 12-myristate 13-acetate, zymosan and beta-glucan. Temperature was shown to have a significant effect on production of reactive radicals as illustrated by a high activity in cells maintained at 15-20 degrees C and a reduced activity at temperature extremes (1, 4 and 30 degrees C). Highest activity was found at a cell concentration of 1 x 10(7) cells mL(-1). Reactivity showed a clear decline when cells were exposed for more than 4 h. Moreover, incubation of cells with inhibitory substances viz., DiMePE2, cortisol and superoxide dismutase decreased the RBA. It is concluded that several biotic and abiotic factors should be taken into account when conducting RBA assays with head kidney leucocytes for elucidation of rainbow trout immune responses. |
| | |
Authors:
|
J K Chettri; L Holten-Andersen; K Buchmann |
Related Documents
:
|
12163898 - The effect of an hiv-1 viral protease inhibitor on staurosporine-induced apoptosis in i... 12602118 - Effects of at1 and at2 receptor blockade on angiotensin ii induced apoptosis of human r... 16216558 - Bmp7 signaling in renal development and disease. 20980738 - The effect of pacap38 on myd88-mediated signal transduction in ischemia-/hypoxia-induce... 17575108 - Modifications enhance the apoptosis-inducing activity of fadd. 15778738 - Expression of functional nk1 receptors in human alveolar macrophages: superoxide anion ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-31 |
Journal Detail:
|
Title: Journal of fish diseases Volume: 33 ISSN: 1365-2761 ISO Abbreviation: J. Fish Dis. Publication Date: 2010 Jul |
Date Detail:
|
Created Date: 2010-07-01 Completed Date: 2010-10-12 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9881188 Medline TA: J Fish Dis Country: England |
Other Details:
|
Languages: eng Pagination: 593-602 Citation Subset: IM |
Affiliation:
|
Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, Frederiksberg C, Denmark. jiwanchettri@rediffmail.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adjuvants, Immunologic
/
pharmacology Animals Anti-Inflammatory Agents / pharmacology Cells, Cultured Leukocytes / drug effects, metabolism* Lymphoid Tissue / cytology Oncorhynchus mykiss* Respiratory Burst / drug effects, physiology* Temperature Time Factors |
| Chemical | |
Reg. No./Substance:
|
0/Adjuvants, Immunologic; 0/Anti-Inflammatory Agents |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Clarifying the Taxonomic Identity of a Phylogenetically Important Group of Eukaryotes: Planomonas is...
Next Document: Expression profiles of toll-like receptors in anterior kidney of channel catfish, Ictalurus punctatu...