Document Detail


Factors influencing helper-independent adeno-associated virus replication.
MedLine Citation:
PMID:  22727829     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The inability of Adeno-Associated Virus (AAV) to replicate on its own is a strong argument in favor of the use of recombinant AAV vectors for in vivo gene transfer. However, some previous studies suggested that AAV may become replication competent in cells exposed to a genotoxic stress even in the absence of co-infection with a helper virus. To comprehensively explore this phenomenon, we examined AAV genome replication in several human cell lines exposed to different genotoxic conditions. We found that all treatments induced only negligible levels of AAV replication never exceeding ten fold above background. Further investigation indicated that induction of helper-independent AAV replication relied on the synergistic contribution of several extrinsic factors linked to the origin of the cell line and the quality of the AAV preparation. These results further support the notion that helper independent AAV replication cannot occur at significant levels in vivo.
Authors:
Armel Nicolas; Nelly Jolinon; Nathalie Alazard-Dany; Véronique Barateau; Alberto L Epstein; Anna Greco; Hildegard Büning; Anna Salvetti
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-22
Journal Detail:
Title:  Virology     Volume:  -     ISSN:  1096-0341     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
Affiliation:
INSERM U758, ENS Lyon, 46 Allée d'Italie, Lyon 69007, France; Ecole Normale Supérieure de Lyon, Lyon 69007, France; Université de Lyon, UCB-Lyon 1, Lyon 69003, France.
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