Document Detail

Factors influencing the electrokinetic dispersion of PAH-degrading bacteria in a laboratory model aquifer.
MedLine Citation:
PMID:  18607588     Owner:  NLM     Status:  MEDLINE    
Despite growing interest in the electro-bioremediation of contaminated soil it is still largely unknown to which degree weak electric fields influence the fate of contaminant-degrading microorganisms in the sub-surface. Here we evaluate the factors influencing the electrokinetic transport and deposition of fluorene-degrading Sphingomonas sp. LB126 in a laboratory model aquifer exposed to a direct current (DC) electric field (1 V cm(-1)) typically used in electro-bioremediation measures. The influence of cell size, cell membrane integrity, cell chromosome contents (all assessed by flow cytometry), cell surface charge and cell hydrophobicity on the spatial distribution of the suspended and matrix-bound cells after 15 h of DC-treatment was evaluated. In presence of DC the cells were predominantly mobilised by electroosmosis to the cathode with an apparent velocity of 0.6 cm h(-1), whereas a minor fraction only of the cells augmented was mobilised to the anode by electrophoresis. Different electrokinetic behaviour of individual cells could be solely attributed to intra-population heterogeneity of the cell surface charge. In the absence of DC by contrast, a Gaussian-type distribution of bacteria around the point of injection was found. DC had no influence on the deposition efficiency, as the glass beads in presence and absence of an electric field retained quasi-equal fractions of the cells. Propidium iodide staining and flow cytometry analysis of the cells indicated the absence of negative influences of DC on the cell wall integrity of electrokinetically mobilised cells and thus point at unchanged physiological fitness of electrokinetically mobilised bacteria.
L Shi; S Müller; H Harms; L Y Wick
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Publication Detail:
Type:  Journal Article     Date:  2008-07-08
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  80     ISSN:  1432-0614     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-08-20     Completed Date:  2008-11-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  507-15     Citation Subset:  IM    
Department of Environmental Microbiology, UFZ Helmholtz Centre for Environmental Research, Leipzig, Germany.
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MeSH Terms
Biodegradation, Environmental
Models, Biological*
Polycyclic Hydrocarbons, Aromatic / metabolism*
Sphingomonas / cytology*,  physiology*
Reg. No./Substance:
0/Polycyclic Hydrocarbons, Aromatic

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