Document Detail

Factors influencing the discrimination and classification of prostate cancer cell lines by FTIR microspectroscopy.
MedLine Citation:
PMID:  19475133     Owner:  NLM     Status:  MEDLINE    
In this study we obtained Fourier transform infrared (FTIR) spectra of fixed prostate cell lines of differing types as well as the primary epithelial cells from benign prostatic hyperplasia (BPH). Results showed that by using multivariate chemometric analysis it was possible to discriminate and classify these cell lines, which gave rise to sensitivity and specificity values of >94% and >98%, respectively. Following on from these results the possible influences of different factors on the discrimination and classification of the prostate cell lines were examined. Firstly, the effect of using different growth media during cell culturing was investigated, with results indicating that this did not influence chemometric discrimination. Secondly, differences in the nucleus-to-cytoplasm (N/C) ratio were examined, and it was concluded that this factor was not the main reason for the discrimination and classification of the prostate cancer (CaP) cell lines. In conclusion, given the fact that neither growth media nor N/C ratio could totally explain the classification it is likely that actual biochemical differences between the cell lines is the major contributing factor.
T J Harvey; E Gazi; A Henderson; R D Snook; N W Clarke; M Brown; P Gardner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-04-17
Journal Detail:
Title:  The Analyst     Volume:  134     ISSN:  1364-5528     ISO Abbreviation:  Analyst     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-05-28     Completed Date:  2010-03-12     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  England    
Other Details:
Languages:  eng     Pagination:  1083-91     Citation Subset:  IM    
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MeSH Terms
Cell Line, Tumor
Cell Nucleus / metabolism
Culture Media
Cytoplasm / metabolism
Discriminant Analysis
Multivariate Analysis
Principal Component Analysis
Prostatic Neoplasms / metabolism,  pathology*
Spectroscopy, Fourier Transform Infrared
Grant Support
G0500966//Medical Research Council
Reg. No./Substance:
0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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