Document Detail

Factors influencing blood supply in wound granuloma quantitated by a new in vivo technique.
MedLine Citation:
PMID:  2463076     Owner:  NLM     Status:  MEDLINE    
A new quantitative assay for measuring angiogenesis in a s.c. located sponge implant in rats is described. Using this model, which detects neovascularization by measuring alterations in 133Xe clearance, it has been shown that the known angiogenic factors, transforming growth factor alpha and tumor necrosis factor alpha, cause maximum vascularization of the sponge to occur by Day 11 postimplantation compared with Days 15 to 17 in control animals. The monokine interleukin 1 alpha is shown to be strongly angiogenic, suggesting that more than one macrophage-derived cytokine may be the active mediator in macrophage-induced angiogenesis. Extracellular matrix proteins appear to play a role in regulating the angiogenic response such that presoaking sponges in laminin (40 micrograms/ml) or fibrinogen (500 micrograms/ml) solutions induced a significant reduction in the time taken to achieve maximum 133Xe clearance values; no such enhancement of neovascularization was observed when sponges were presoaked in type IV collagen (100 micrograms/ml) solution. The assay described here, which is reproducible, objective, and quantitative, should be of considerable use in elucidating the molecular basis of angiogenesis regulation.
V Mahadevan; I R Hart; G P Lewis
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cancer research     Volume:  49     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1989 Jan 
Date Detail:
Created Date:  1989-02-22     Completed Date:  1989-02-22     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  415-9     Citation Subset:  IM    
Department of Pharmacology, Royal College of Surgeons of England, London, United Kingdom.
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MeSH Terms
Extracellular Matrix / metabolism
Granuloma / pathology*
Interleukin-1 / pharmacology
Neovascularization, Pathologic*
Rats, Inbred Strains
Serum Albumin, Bovine
Transforming Growth Factors / pharmacology
Tumor Necrosis Factor-alpha / pharmacology
Wound Healing*
Reg. No./Substance:
0/Interleukin-1; 0/Laminin; 0/Serum Albumin, Bovine; 0/Tumor Necrosis Factor-alpha; 76057-06-2/Transforming Growth Factors; 9001-32-5/Fibrinogen; 9007-34-5/Collagen

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