Document Detail


Factors contributing to false-negative and potential false-negative cytology reports in SurePath™ liquid-based cervical cytology.
MedLine Citation:
PMID:  22775526     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
N. Gupta, D. John, N. Dudding, J. Crossley and J. H. F. Smith Factors contributing to false-negative and potential false-negative cytology reports in SurePath™liquid-based cervical cytology Objectives:  The characteristics of false-negative conventional cervical cytology smears have been well documented, but there is limited literature available for liquid-based cytology (LBC), especially SurePath™ samples. We aimed to assess the characteristics of false-negative SurePath LBC samples. Methods:  Over a period of 5 years, an audit of false-negative reports in SurePath cervical cytology was undertaken. In a workload of 183, 112 samples, 481 (0.3%) false negatives were identified using two routes: those detected by routine laboratory internal quality control (rapid pre-screening) (n = 463) and those reported as normal (true false negatives) with concurrent high-grade cervical histology (n = 18). Ninety-five false-negative cases with a subsequent biopsy reported as at least cervical intraepithelial neoplasia grade 2 (CIN2+) were reviewed for a number of different cytomorphological features. Results:  Of 95 samples with subsequent CIN2+, 30.5% predominately contained microbiopsies/hyperchromatic crowded cell groups (HCGs), 27.3% sparse dyskarytotic cells, 4.2% pale cell dyskaryosis, 6.3% small dyskaryotic cells; 3.2% were misinterpreted cells, 8.4% contained other distracting cells, 7.4% were low contrast, 5.3% were unexplained and 7.4% were true negatives. The mean number of microbiopsies/HCGs in that category was 4.6. The mean number of abnormal cells in the sparse dyskaryotic cell category was 13.8. Conclusions:  Microbiopsies/HCGs were the commonest reason for false negatives. They were usually present in sufficient numbers to be detected but interpretation could be problematic. Dispersed single abnormal cells were usually not identified because of their scarcity or the presence of distracters.
Authors:
N Gupta; D John; N Dudding; J Crossley; J H F Smith
Related Documents :
20639786 - Mdm2-positive atypical lipomatous neoplasm/well-differentiated liposarcoma versus spind...
6501426 - Map 4: a microtubule-associated protein specific for a subset of tissue microtubules.
15773896 - The maintenance of the endoplasmic reticulum network is regulated by p47, a cofactor of...
9067616 - Development of polarity in human erythroleukemia cells: roles of membrane ruffling and ...
12960316 - An il-2-dependent switch between cd95 signaling pathways sensitizes primary human t cel...
23787896 - Isolation, culture and evaluation of multilineage-differentiating stress-enduring (muse...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-9
Journal Detail:
Title:  Cytopathology : official journal of the British Society for Clinical Cytology     Volume:  -     ISSN:  1365-2303     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9010345     Medline TA:  Cytopathology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 Blackwell Publishing Ltd.
Affiliation:
Department of Histopathology & Cytology, Royal Hallamshire Hospital, Sheffield, UK.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Clinical development of IMOJEV ®--a recombinant Japanese encephalitis chimeric vaccine (JE-CV).
Next Document:  Assessment of Marginal Adaptation After Apicoectomy and Apical Sealing with Nd:YAG Laser.