Document Detail


Factors affecting the survivability of bovine oocytes vitrified in droplets.
MedLine Citation:
PMID:  11101028     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Vitrification of bovine oocytes performed using the traditional, in straw system has not given satisfactory results. Although an alternative approach based on minimizing the volume of the vitrified sample has recently resulted in a much more promising survival rate of vitrified oocytes, we attempted to examine some additional factors influencing the survival and subsequent fertilization and development rates of bovine oocytes subjected to vitrification according to the minimum drop size approach. In total, 748 bovine, in vitro matured oocytes were vitrified using VS14 vitrification solution, containing 5.5-M ethylene glycol and 1.0-M sucrose after different pre-equilibration and equilibration protocols performed at 35 degrees to 37 degrees C. Experiment 1 showed no significant toxic effect during pre-equilibration treatments of oocytes in 2%, 4% or 6% ethylene glycol solutions, except the lower cleavage rate of oocytes exposed to 6% ethylene glycol (77.2% vs. 93.9% in control, P< 0.05). In Experiment 2, 12 to 15 min of pre-equilibration treatments in 0%, 1% or 2% ethylene glycol solutions were tested, followed by 30 or 45 sec of equilibration in VS 14 solution and vitrification in droplets of medium dropped directly into liquid nitrogen. The development rate of vitrified oocytes to the blastocyst stage tended to be higher after 30-sec equilibration treatment (9.5%, 13.9% and 13.8% in groups of oocytes pre-equilibrated in 0%, 1% or 2% ethylene glycol solutions, respectively). Experiment 3 tested pre-equilibration treatments in 0%, 1%, 2%, 3%, 4%, 5% or 6% ethylene glycol solutions, followed by 30-sec equilibration and vitrification in droplets. The highest cleavage, blastocyst and hatched blastocyst rates, which were not significantly different from control, were achieved in a group of oocytes pre-equilibrated in 3% ethylene glycol solution (76%, 30% and 15% vs. 89%, 42% and 21% in control, respectively). A healthy calf was born on Feb 22 1999, after transfer of 4 morula/blastocyst stage embryos developed from oocytes vitrified in droplets after pre-equilibration in 3% ethylene glycol solution. We conclude that gentle pre-equilibration of bovine oocytes in diluted, 3% ethylene glycol solution is an important factor improving the effectiveness of vitrification in droplets of bovine oocytes.
Authors:
K Papis; M Shimizu; Y Izaike
Related Documents :
15290858 - Preparation and drug loading of poly(ethylene glycol)-block-poly(epsilon-caprolactone) ...
16788218 - Fatal aspiration of polyethylene glycol solution.
8031988 - Protein adsorption of poly(ether urethane) surfaces modified by amphiphilic and hydroph...
17118588 - Evaluation of bioadhesive potential and intestinal transport of pegylated poly(anhydrid...
17638348 - Surface modified polypropylene pipette tips packed with a monolithic plug of adsorbent ...
6502788 - New aspects in the treatment of water hemlock poisoning.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Theriogenology     Volume:  54     ISSN:  0093-691X     ISO Abbreviation:  Theriogenology     Publication Date:  2000 Sep 
Date Detail:
Created Date:  2001-01-18     Completed Date:  2001-01-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  651-8     Citation Subset:  IM    
Affiliation:
lnstitute of Genetics and Animal Breeding, Jastrzebiec, Mroków, Poland. kpapis@yahoo.com
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Cattle / physiology*
Cryopreservation / methods,  veterinary*
Cryoprotective Agents / pharmacology*
Embryo Transfer / veterinary
Ethylene Glycol / pharmacology
Female
Fertilization in Vitro / veterinary
Male
Oocytes / physiology*
Pregnancy
Random Allocation
Time Factors
Chemical
Reg. No./Substance:
0/Cryoprotective Agents; 107-21-1/Ethylene Glycol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Epidemiology and predictors of infant morbidity in rural Malawi.
Next Document:  Culture of in vitro produced bovine zygotes in vitro vs in vivo: implications for early embryo devel...