| Factors affecting the phosphorylation of a 41,000 dalton protein of hippocampal subcellular fractions. | |
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MedLine Citation:
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PMID: 6733499 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The phosphorylation of the alpha-subunit of mitochondrial pyruvate dehydrogenase may be involved in the development of long-term potentiation in the hippocampus. Study of this hypothesis is hampered by variability in the incorporation of 32P into pyruvate dehydrogenase of hippocampal subcellular preparations, in vitro. 32P from [gamma-32P]ATP was incorporated into pyruvate dehydrogenase present in mitochondria and in a membrane-enriched synaptic particulate fraction from hippocampus. However, the presence of the synaptic fraction decreased isotopic labeling of the mitochondrial protein. This effect was not due to inhibition of the protein kinase or activation of a protein phosphatase, but the rate of ATP hydrolysis was found to be higher in the synaptic fraction than in the mitochondria (34 nmol/mg protein/min vs 14 nmol/mg protein/min). These data raise a variety of questions about the interpretation of the in vitro phosphorylation assay. It is concluded that variability in in vitro labeling can be minimized if the effect of ATP hydrolysis is diminished by use of a higher concentration of ATP. In addition, these data indicate that quantitative comparisons of the in vitro phosphorylation of diverse subcellular preparations must take into account differential rates of ATP hydrolysis. |
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Authors:
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D B Hoch; J E Wilson; R J Dingledine |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Brain research Volume: 302 ISSN: 0006-8993 ISO Abbreviation: Brain Res. Publication Date: 1984 Jun |
Date Detail:
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Created Date: 1984-08-20 Completed Date: 1984-08-20 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0045503 Medline TA: Brain Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 117-24 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism Animals Hippocampus / enzymology* Kinetics Male Mitochondria / enzymology Molecular Weight Phosphorus Radioisotopes / diagnostic use Phosphorylation Pyruvate Dehydrogenase Complex / isolation & purification, metabolism* Rats Rats, Inbred Strains Subcellular Fractions / enzymology Synaptosomes / enzymology |
| Grant Support | |
ID/Acronym/Agency:
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NS-15334/NS/NINDS NIH HHS; T32-MH14277/MH/NIMH NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Phosphorus Radioisotopes; 0/Pyruvate Dehydrogenase Complex; 56-65-5/Adenosine Triphosphate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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