Document Detail

Factors affecting phaeomelanin production by a melanin-producing (mel) mutant of Vibrio cholerae.
MedLine Citation:
PMID:  7333674     Owner:  NLM     Status:  MEDLINE    
In a previous study we isolated melanin-producing (mel) mutants of Vibrio cholerae and demonstrated that production of melanin during growth on solid media was stimulated by L-tyrosine and L-cysteine. In the studies reported here we analyzed factors that affected melanin production in liquid media and determined the abilities of radioactively labeled amino acids to serve as precursors for the formation of melanin by V. cholerae. Radioactivity from L-cysteine and from L-tyrosine was preferentially incorporated into partially purified melanin, providing further evidence for production of phaeomelanin by V. cholerae. Cuprous ions stimulated production of melanin by V. cholerae in defined liquid medium, but melanin formation was inhibited by high concentrations of L-cysteine or thiouracil. The inhibition of melanin formation by sulfhydryl compounds is most likely due to their ability to bind copper ions that are essential for tyrosinase activity.
B E Ivins; R K Holmes
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  34     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1981 Dec 
Date Detail:
Created Date:  1982-05-12     Completed Date:  1982-05-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  895-9     Citation Subset:  IM    
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MeSH Terms
Copper / pharmacology
Cysteine / pharmacology
Melanins / biosynthesis*
Vibrio cholerae / genetics,  metabolism*
Reg. No./Substance:
0/Melanins; 12627-61-1/phaeomelanin; 52-90-4/Cysteine; 7440-50-8/Copper

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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