Document Detail


Factors affecting outcome after ICSI with spermatozoa retrieved from cryopreserved testicular tissue in non-obstructive azoospermia.
MedLine Citation:
PMID:  15901451     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
There is a lack of data regarding variables affecting the treatment outcome for non-obstructive azoospermia when spermatozoa from cryopreserved testicular specimens are utilized for ICSI. The objective of the present retrospective analysis was to investigate the effect of various parameters on treatment outcome in such cases. One hundred and sixty-five couples with non-obstructive azoospermic males undergoing a total of 297 cycles were included. In all cases the testicular tissue retrieved by multiple open-biopsy testicular sperm extraction was stored in liquid nitrogen and, after thawing, only mature spermatozoa were used for ICSI. When no motile spermatozoa were recovered, immotile spermatozoa were used. In 159 cycles, motile spermatozoa were utilized for ICSI, while in 138 cycles immotile spermatozoa were utilized. Higher normal fertilization rate (60.4 +/- 3.1 versus 51.3 +/- 1.6%, P < 0.05), number of embryos transferred (2.8 +/- 0.06 versus 2.6 +/- 0.04, P < 0.05), modified cumulative embryo score (31.2 +/- 1.6 versus 23.9 +/- 0.8, P < 0.001), and proportion of motile spermatozoa injected (67.8 versus 49.8%, P < 0.05) were observed in cycles that resulted in clinical pregnancies. Binary logistic regression analysis showed that sperm motility (odds ratio 2.06, 95% CI 1.1-3.9, P < 0.05), but not woman's age, number of treatment cycle, type of GnRH-analogue used for pituitary suppression, number of oocytes retrieved or number of embryos transferred was a significant determinant of the likelihood of clinical pregnancy. In conclusion, sperm motility after freeze/thawing of testicular tissue is the major determinant of the success of ICSI in non-obstructive azoospermia.
Authors:
Konstantinos Dafopoulos; Georg Griesinger; Askan Schultze-Mosgau; Yasser Orief; Beate Schöpper; Nikos Nikolettos; Klaus Diedrich; Safaa Al-Hasani
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  10     ISSN:  1472-6483     ISO Abbreviation:  Reprod. Biomed. Online     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-05-19     Completed Date:  2005-06-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  England    
Other Details:
Languages:  eng     Pagination:  455-60     Citation Subset:  IM    
Affiliation:
Department of Gynecology and Obstetrics, University of Schleswig-Holstein, Campus Lubeck, Ratzeburger Allee 160, 23538 Lubeck, Germany.
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MeSH Terms
Descriptor/Qualifier:
Adult
Cryopreservation*
Embryo Transfer
Female
Fertilization
Humans
Logistic Models
Male
Oligospermia* / physiopathology
Pregnancy
Pregnancy Rate
Retrospective Studies
Sperm Injections, Intracytoplasmic*
Sperm Motility
Spermatozoa*
Testis*
Tissue and Organ Harvesting*
Treatment Outcome

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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