Document Detail

Factors affecting measurement of total alkaline phosphatase activity in human serum, especially wavelength accuracy.
MedLine Citation:
PMID:  657484     Owner:  NLM     Status:  MEDLINE    
Serum alkaline phosphatase estimations by the Selected Method [Clin. Chem. 21, 1988 (1975)] are very sensitive to small errors in the wavelength setting of the spectrophotometer. With a setting error as small as 3 nm, results can be too low by as much as 23%. The product of the reaction, 4-nitrophenol, can be prepared in very pure form and can be used to check both the wavelength and absorbance accuracy of a spectrophotometer. The molar absorptivity for 4-nitrophenol obtained in the instrument being used should be used in calculating alkaline phosphatase activities.
J A Lott; K Turner; J Scott
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Clinical chemistry     Volume:  24     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1978 Jun 
Date Detail:
Created Date:  1978-08-28     Completed Date:  1978-08-28     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  938-41     Citation Subset:  IM    
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MeSH Terms
Alkaline Phosphatase / blood*
Evaluation Studies as Topic
Spectrophotometry / methods
Reg. No./Substance:
EC Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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