Document Detail


Factors affecting the irreversible attachment of Pseudomonas aeruginosa to stainless steel.
MedLine Citation:
PMID:  6423248     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To better understand the interaction between bacteria and surfaces, we studied the irreversible attachment of Pseudomonas aeruginosa to a common surfacing material. When brought into contact with the steel, cells began to attach in less than 1 min and the number adhering increased with time. An important physiological variable in attachment was cell motility since adherence decreased at least 90% when flagella were removed by blending. This treatment was shown to be effective because it caused motility loss and not because it removed a structure necessary for adherence. Cell viability was less important since adherence decreased only 50% when the number of viable cells was reduced 4.7 logs by heating or formaldehyde treatment. Significant environmental variables included turbulence and ionic strength. Attachment of motile cells was reduced 90% by agitation, although agitation had little effect on adherence of nonmotile cells. Both motile and nonmotile cells adhered poorly in distilled water with attachment increasing as CaCl2 or NaCl concentration increased to 10 mM. At 100 mM, attachment decreased. Viable cells, both motile and nonmotile, adhered best at a pH of 7 to 8, whereas nonviable cells attached most rapidly at a low pH.
Authors:
P M Stanley
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Canadian journal of microbiology     Volume:  29     ISSN:  0008-4166     ISO Abbreviation:  Can. J. Microbiol.     Publication Date:  1983 Nov 
Date Detail:
Created Date:  1984-05-21     Completed Date:  1984-05-21     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372707     Medline TA:  Can J Microbiol     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  1493-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adhesiveness
Calcium Chloride / pharmacology
Flagella / physiology
Formaldehyde / pharmacology
Hot Temperature
Hydrogen-Ion Concentration
Movement
Osmolar Concentration
Pseudomonas aeruginosa / growth & development,  physiology*
Sodium Chloride / pharmacology
Stainless Steel*
Chemical
Reg. No./Substance:
10043-52-4/Calcium Chloride; 12597-68-1/Stainless Steel; 50-00-0/Formaldehyde; 7647-14-5/Sodium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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