Document Detail


Factors affecting the hydroxycinnamate decarboxylase/vinylphenol reductase activity of dekkera/brettanomyces: application for dekkera/brettanomyces control in red wine making.
MedLine Citation:
PMID:  19200101     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The growth of Dekkera/Brettanomyces yeasts during the ageing of red wines-which can seriously reduce the quality of the final product-is difficult to control. The present study examines the hydroxycinnamate decarboxylase/vinylphenol reductase activity of different strains of Dekkera bruxellensis and Dekkera anomala under a range of growth-limiting conditions with the aim of finding solutions to this problem. The yeasts were cultured in in-house growth media containing different quantities of growth inhibitors such as ethanol, SO(2), ascorbic acid, benzoic acid and nicostatin, different sugar contents, and at different pHs and temperatures. The reduction of p-coumaric acid and the formation of 4-ethylphenol were periodically monitored by HPLC-PDA. The results of this study allow the optimization of differential media for detecting/culturing these yeasts, and suggest possible ways of controlling these organisms in wineries.
Authors:
S Benito; F Palomero; A Morata; F Calder?n; J A Su?rez-Lepe
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of food science     Volume:  74     ISSN:  1750-3841     ISO Abbreviation:  J. Food Sci.     Publication Date:    2009 Jan-Feb
Date Detail:
Created Date:  2009-02-09     Completed Date:  2009-05-18     Revised Date:  2010-05-24    
Medline Journal Info:
Nlm Unique ID:  0014052     Medline TA:  J Food Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  M15-22     Citation Subset:  IM    
Affiliation:
Dpto. Tecnolog?a de Alimentos, Univ. Polit?cnica de Madrid, Spain.
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MeSH Terms
Descriptor/Qualifier:
Brettanomyces / enzymology*,  growth & development
Carboxy-Lyases / metabolism*
Chromatography, High Pressure Liquid
Coumaric Acids / analysis,  metabolism
Culture Media / chemistry
Dekkera / enzymology*,  growth & development
Fermentation
Food Contamination / analysis*
Hydrogen-Ion Concentration
Phenols / analysis,  metabolism*
Temperature
Wine / microbiology*
Chemical
Reg. No./Substance:
0/Coumaric Acids; 0/Culture Media; 0/Phenols; 2628-17-3/4-vinylphenol; EC 4.1.1.-/Carboxy-Lyases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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