| Factors affecting the esterification of lauric acid using an immobilized biocatalyst: Enzyme characterization and studies in a well-mixed reactor. | |
| | |
MedLine Citation:
|
PMID: 18623264 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
The esterification of lauric acid with geraniol catalyzed by the commercially immobilized lipase preparation from Mucor miehei, Lipozyme(R), was studied in well-stirred flasks. The enzyme support was characterized in terms of its internal and external surface area, protein location, and protein content. It was found that the enzyme was mainly located on the external surface of the support, therefore, internal diffusional limitations were not important. It was also shown that the protein content of the support depends on the size of the particle, with smaller particles containing higher amounts of protein per unit weight. Under the conditions studied, the reaction was not under external mass transfer limitations, and the initial reaction rate depended on the size of the support particles. This was mainly due to the different protein contents on the support as a function of particle size and not to internal or external mass transfer limitations. Also, it was found that the inhibition exerted by water was predominantly a physical effect due to its accumulation around the enzyme. It was also found that the reaction was substrate inhibited by lauric acid, but not by geraniol. (c) 1995 John Wiley & Sons, Inc. |
| | |
Authors:
|
F V Lima; D L Pyle; J A Asenjo |
Related Documents
:
|
8061224 - Glycolytic ph oscillations in a flow reactor. 14665734 - Production of l-dopa by tyrosinase immobilized on modified polystyrene. 1515474 - Activating hydroxyl groups of polymeric carriers using 4-fluorobenzenesulfonyl chloride. 18439774 - Concanavalin a layered calcium alginate-starch beads immobilized beta galactosidase as ... 8302274 - Generation of reactive oxygen intermediates by human liver microsomes in the presence o... 8190704 - Lipoperoxidative damage on lead exposure in rat brain and its implications on membrane ... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Biotechnology and bioengineering Volume: 46 ISSN: 0006-3592 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 1995 Apr |
Date Detail:
|
Created Date: 2008-07-14 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: United States |
Other Details:
|
Languages: eng Pagination: 69-79 Citation Subset: - |
Affiliation:
|
Biotechnology and Biochemical Engineering Group, University of Reading, Reading RG6 2AP, United Kingdom. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Genetically engineered charge modifications to enhance protein separation in aqueous two-phase syste...
Next Document: Degradation of quinoline by immobilized Comamonas acidovorans in a three-phase airlift reactor.