Document Detail


Factors affecting the efficacy of pressure inactivation of Escherichia coli O157:H7 on alfalfa seeds and seed viability.
MedLine Citation:
PMID:  19339075     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The application of high hydrostatic pressure technology as a seed decontamination technology was evaluated. Alfalfa seeds inoculated with approximately 10(5) CFU/g of Escherichia coli O157:H7 were subjected to oscillatory pressure treatments at 600 MPa and 20 degrees C for up to five cycles with a holding time of 2 min/cycle. However, oscillatory pressurization was not able to eliminate E. coli O157:H7. The application of pressure treatment at 600 MPa for 2 min at 20 degrees C in the presence of chemicals such as calcium hypochlorite, calcium hydroxide, lactic acid or sodium acid sulfate was subsequently investigated and it was demonstrated that this "multiple hurdle" approach was unable to decontaminate alfalfa seeds. Soaking seeds prior to pressure treatment was found to play a critical role on enhancing the pressure inactivation of E. coli O157:H7; seeds soaked in water for 60 min followed by treatment at 600 MPa for 2 min at 20 degrees C were decontaminated and had a germination rate of 91% which was 4% lower than that of the untreated seeds (not statistically significant, P>0.05). It was further demonstrated that a process involving soaking seeds in water for > or = 10 min followed by treatment at 600 MPa for 15 min at 20 degrees C was equally effective with respect to E. coli O157:H7 elimination and viability retention of the seeds.
Authors:
Hudaa Neetoo; Mu Ye; Haiqiang Chen
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Publication Detail:
Type:  Journal Article     Date:  2009-03-12
Journal Detail:
Title:  International journal of food microbiology     Volume:  131     ISSN:  1879-3460     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-20     Completed Date:  2009-08-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  218-23     Citation Subset:  IM    
Affiliation:
Department of Animal and Food Sciences, University of Delaware, Newark, DE 19716-2150, USA.
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MeSH Terms
Descriptor/Qualifier:
Calcium Compounds
Calcium Hydroxide
Disinfection / methods*
Escherichia coli O157*
Food Handling / methods
Food Microbiology*
Food Preservation / methods
Germination
Hydrostatic Pressure
Lactic Acid
Medicago sativa / microbiology*
Seeds / microbiology*
Water*
Chemical
Reg. No./Substance:
0/Calcium Compounds; 1305-62-0/Calcium Hydroxide; 50-21-5/Lactic Acid; 7732-18-5/Water; 7778-54-3/calcium hypochlorite

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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