| Factors affecting the binding of polycyclic aromatic hydrocarbons to human embryo cells, and transformable and non-transformable hamster embryo cells. | |
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MedLine Citation:
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PMID: 100230 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of various factors, including population doubling number, percent of confluence, serum concentration and storage in liquid nitrogen on the binding of several polycyclic aromatic hydrocarbons to human and hamster embryo cells were studied. The binding of 7,12-dimethylbenz[a]-anthracene (DMBA) to hamster embryo cells DNA, RNA and protein was maximal after 22 h of treatment. In contrast, binding to human embryo cell macromolecules increased for at least 55 h. Treatment of hamster embryo cells at 100% confluence resulted in much less binding than treatment at 70% confluence, whereas with human embryo cells the binding increased, or remained constant, following treatment at the greater confluence. The transforming frequency of hamster embryo cells decreases with increasing population doubling number. Accordingly, we found that the binding of DMBA to hamster embryo DNA, RNA and protein decreased approximately 100-fold between population doubling numbers 8 and 20. In transformable cell cultures, DMBA was bound to hamster embryo cell DNA to a greater extent than to RNA or protein. The binding of DMBA to nucleic acids was much greater than binding by either dibenz[a,h]anthracene (DB[a,h]A) or dibenz-[a,c]anthracene (DB[a,c]A), both of which had low binding values at all population doubling numbers tested. Therefore, the best correlation of binding with carcinogenicity and transforming activity was observed with DMBA. Storage of hamster embryo cells in liquid nitrogen did not alter their binding characteristics. Binding of all three hydrocarbons to human embryo cell nucleic acids was low during all population doubling numbers studied, while binding to cellular protein increased until population doubling number 70 and then decreased sharply. |
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Authors:
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R Raineri; J A Poiley; T Hillesund; R J Pienta |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Chemico-biological interactions Volume: 22 ISSN: 0009-2797 ISO Abbreviation: Chem. Biol. Interact. Publication Date: 1978 Sep |
Date Detail:
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Created Date: 1978-12-20 Completed Date: 1978-12-20 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0227276 Medline TA: Chem Biol Interact Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 257-70 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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9,10-Dimethyl-1,2-benzanthracene
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metabolism Animals Benz(a)Anthracenes / metabolism Cell Division Cell Transformation, Neoplastic Cells, Cultured Cricetinae DNA / metabolism Embryo, Mammalian / cytology, metabolism* Humans Mesocricetus Polycyclic Compounds / metabolism* Protein Binding RNA / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Benz(a)Anthracenes; 0/Polycyclic Compounds; 57-97-6/9,10-Dimethyl-1,2-benzanthracene; 63231-63-0/RNA; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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