| Factors Influencing the Oxygen Consumption Rate of Aortic Valve Interstitial Cells: Application to Tissue Engineering. | |
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MedLine Citation:
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PMID: 19196141 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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This study investigated the factors influencing the oxygen metabolism of aortic valve interstitial cells (VICs). Porcine VICs in cell suspension at different passages, and adhered to coverslips at different confluencies, as well as fresh porcine valve leaflets, were incubated in an oxygen respiration chamber at 37 degrees C in Dulbecco's modified Eagle's medium. The consumption rates at different oxygen concentrations were evaluated based on the Michaelis-Menten equation, and the corresponding maximum consumption rate (V(max)) and the Michaelis-Menten equation constant K(m) were determined. In all cases, the oxygen consumption rate was relatively constant until the concentration dropped to 5% (v/v). The metabolic activity of VICs in terms of oxygen consumption was dependent upon their in vitro passage number and proliferation status. These findings will provide valuable input to the selection of VICs with respect to their age and proliferation status for tissue engineering applications, as well as important input parameters for developing computational models of oxygen transport and optimization of the bioreactor conditions for heart valve tissue engineering. |
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Authors:
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Ling Wang; Stacy-Paul Wilshaw; Sotirios Korossis; John Fisher; Zhongmin Jin; Eileen Ingham |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2009-1-14 |
Journal Detail:
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Title: Tissue engineering. Part A Volume: - ISSN: 1937-335X ISO Abbreviation: - Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2009-2-6 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101466659 Medline TA: Tissue Eng Part A Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Institute of Medical and Biological Engineering, University of Leeds , Leeds, United Kingdom . |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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