Document Detail


Factors Affecting Transformation of Pasteurella novicida.
MedLine Citation:
PMID:  16559109     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
The requirements and characteristics of Pasteurella novicida transformations in liquid suspensions were studied. Transformation frequencies of 0.1 to 0.3% were routinely obtained when recipient cells were harvested from 16-hr agar plates and higher than 1% when logarithmic-phase broth-grown cells were used. Calcium ions were essential for transformations. The deoxyribonucleic acid dose response curve, kinetics of transformation, and pH optimum for transformations were similar to those of other bacterial transformation systems. The genetic relatedness of P. novicida and P. tularensis was established by transforming P. novicida mutants with deoxyribonucleic acid extracted from P. tularensis.
Authors:
F J Tyeryar; W D Lawton
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  104     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1970 Dec 
Date Detail:
Created Date:  2010-06-29     Completed Date:  2010-06-29     Revised Date:  2010-09-14    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1312-7     Citation Subset:  -    
Affiliation:
Biological Sciences Laboratories, Department of the Army, Fort Detrick, Frederick, Maryland 21701.
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