Document Detail


Factors affecting tissue culture and transplantation using omentum.
MedLine Citation:
PMID:  20559129     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In tissue engineering, a stable tissue layer and blood vessels are required for complete tissue formation, to provide structural strength and thickness and to supply oxygen and various nutrients. However, this has not been achieved using in vitro tissue-engineered culture techniques, thus many tissue engineering studies of trachea, bladder, and intestine reconstruction have used omentum. However, many factors critical to cell culture and transplantation using omentum have not yet been studied. For these reasons, we conducted a study of artificial trachea reconstruction in dogs using a poly(lactic-co-glycolic acid) (PLGA) porous scaffold, polypropylene prosthesis, and PKH26-labeled cells. We analyzed factors affecting tissue-engineered reconstruction using omentum, such as cell distribution and formation of cell layer and stability of transplant shape on omentum. As a result, we classified failure factors for tissue-engineered application of omentum and suggested three considerations for effective use of omentum in tissue engineering. Our observations may aid in the design and execution of future studies of omentum usage in tissue engineering.
Authors:
Jin Hoon Kim; Jhingook Kim; Won Ho Kong; Soo Won Seo
Related Documents :
17449879 - Vaginoplasty using autologous in vitro cultured vaginal tissue in a patient with mayer-...
16661709 - Transfer ribonucleic acid modification and its relationship to tumorous and nontumorous...
4631989 - Studies on gonococcus infection. iv. pili: their role in attachment of gonococci to tis...
10888299 - Growth factor delivery for tissue engineering.
3456459 - Monoclonal antibody defining fibroblasts appearing in fetal and neoplastic tissues.
67209 - Lysis of human normal and sarcoma cells in tissue culture by normal human serum: implic...
3828789 - Biochemical and morphological studies on gaba neurons in reaggregate culture.
2547739 - Technetium metabolism by lactating goats.
16411609 - Polyaniline, an electroactive polymer, supports adhesion and proliferation of cardiac m...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  ASAIO journal (American Society for Artificial Internal Organs : 1992)     Volume:  56     ISSN:  1538-943X     ISO Abbreviation:  ASAIO J.     Publication Date:    2010 Jul-Aug
Date Detail:
Created Date:  2010-07-01     Completed Date:  2010-09-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9204109     Medline TA:  ASAIO J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  349-55     Citation Subset:  IM    
Affiliation:
SungKyunKwan University, School of Medicine, Biomedical Engineering Interdisciplinary Course, Seoul, Korea.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Artificial Organs*
Dogs
Lactic Acid
Omentum*
Polyglycolic Acid
Tissue Culture Techniques / methods*
Tissue Engineering / methods*
Trachea*
Chemical
Reg. No./Substance:
0/polylactic acid-polyglycolic acid copolymer; 26009-03-0/Polyglycolic Acid; 50-21-5/Lactic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Tissue Doppler Measurements Correlate with Central Venous Pressure in Pediatric Patients After Cardi...
Next Document:  Modeling Oxygen Transport in a Cylindrical Bioartificial Pancreas.