Document Detail

Factorial analysis of tricarboxylic acid cycle intermediates for optimization of zeaxanthin production from Flavobacterium multivorum.
MedLine Citation:
PMID:  14962143     Owner:  NLM     Status:  MEDLINE    
AIMS: To study the effect of intermediates of the tricarboxylic acid (TCA) cycle on the production of zeaxanthin from Flavobacterium multivorum in order to optimize production of this xanthophyll carotenoid. METHODS AND RESULTS: The concentration of selected TCA cycle intermediates (malic acid, isocitric acid and alpha-ketoglutarate) was optimized in shake flask culture, using a statistical two-level, three-variable factorial approach. The carotenoid production profile was also studied in the optimized medium at various growth phases. Optimized medium resulted in a sixfold increase in volumetric production of zeaxanthin (10.65 +/- 0.63 microg ml-1) using malic acid (6.02 mm), isocitric acid (6.20 mm) and alpha-ketoglutarate (0.02 mm). The majority of zeaxanthin was produced in the late logarithmic growth phase whereas a substantial amount of beta-cryptoxanthin and beta-carotene were observed in the early logarithmic phase. SIGNIFICANCE AND IMPACT OF THE STUDY: This study demonstrates improvement of zeaxanthin production from F. multivorum which might aid in the commercialization of zeaxanthin production from this microbe.
P Bhosale; A J Larson; P S Bernstein
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of applied microbiology     Volume:  96     ISSN:  1364-5072     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2004  
Date Detail:
Created Date:  2004-02-13     Completed Date:  2004-06-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  623-9     Citation Subset:  IM    
Department of Ophthalmology and Visual Sciences, University of Utah School of Medicine, Salt Lake City, UT 84132, USA.
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MeSH Terms
Chromatography, High Pressure Liquid
Citric Acid Cycle*
Culture Media
Dietary Supplements
Flavobacterium / growth & development,  metabolism*
Isocitrates / metabolism
Ketoglutaric Acids / metabolism
Macular Degeneration / drug therapy
Malates / metabolism
Neoplasms / drug therapy
beta Carotene / analogs & derivatives,  analysis*,  biosynthesis,  therapeutic use
Grant Support
Reg. No./Substance:
0/Culture Media; 0/Isocitrates; 0/Ketoglutaric Acids; 0/Malates; 0/Xanthophylls; 144-68-3/zeaxanthin; 320-77-4/isocitric acid; 328-50-7/alpha-ketoglutaric acid; 6915-15-7/malic acid; 7235-40-7/beta Carotene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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