| Facility changes mediated by cAMP in the bovine anterior segment in vitro. | |
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MedLine Citation:
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PMID: 9068827 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to investigate the influence of substances that increase intracellular cAMP levels on the aqueous humor outflow facility (C) of isolated bovine anterior segments. Anterior segments were perfused in vitro at a constant pressure of 10 mmHg for 270 min with a general protocol as follows: 90 min control perfusion with DMEM, 90 min of experimental perfusion with DMEM containing the test drug(s), and 90 min of postdrug-perfusion with DMEM. C was calculated as the ratio between the rate of medium inflow (microliter/min) and the perfusion pressure (mmHg). Anterior segments can be perfused in vitro for up to 5 hr without significantly modifying their C. The addition of epinephrine, forskolin, dibutyryl-cAMP or isobutylmethylxanthine to the control perfusion medium elicited a significant increase of C. If, during isobutylmethylxanthine perfusion, forskolin or epinephrine was added, C increased significantly. Finally, perfusion with indomethacin prior to addition of epinephrine prevented the increase of C induced by epinephrine. Epinephrine, the adenylate cyclase activator forskolin, the cAMP analog dibutyryl-cAMP, and the phosphodiesterase inhibitor isobutylmethylxanthine all increase aqueous facility. It seems reasonable to suspect that the cAMP system is involved in epinephrine's effects on bovine trabecular meshwork cells. Moreover, the complete inhibition by indomethacin of the outflow facility increase induced by epinephrine suggests that prostaglandins may be involved in the outflow facility mechanisms related to adrenoreceptor stimulation of trabecular meshwork cells. |
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Authors:
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R Gilabert; X Gasull; J Palés; C Belmonte; M V Bergamini; A Gual |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Vision research Volume: 37 ISSN: 0042-6989 ISO Abbreviation: Vision Res. Publication Date: 1997 Jan |
Date Detail:
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Created Date: 1997-04-09 Completed Date: 1997-04-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0417402 Medline TA: Vision Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 9-15 Citation Subset: IM |
Affiliation:
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Laboratori de Neurofisiologia, Falcultat de Medicina-Fundacio Clinic, Universitat de Barcelona, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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1-Methyl-3-isobutylxanthine
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pharmacology Animals Anterior Chamber / drug effects, metabolism* Anti-Inflammatory Agents, Non-Steroidal / pharmacology Aqueous Humor / drug effects, physiology* Bucladesine / pharmacology Cattle Cyclic AMP / metabolism*, physiology Drug Antagonism Epinephrine / pharmacology Forskolin / pharmacology Indomethacin / pharmacology Mydriatics / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Anti-Inflammatory Agents, Non-Steroidal; 0/Mydriatics; 28822-58-4/1-Methyl-3-isobutylxanthine; 362-74-3/Bucladesine; 51-43-4/Epinephrine; 53-86-1/Indomethacin; 60-92-4/Cyclic AMP; 66428-89-5/Forskolin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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