| Facilitation of dissociation reaction of nucleotides bound to Mycobacterium tuberculosis DnaA. | |
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MedLine Citation:
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PMID: 18296714 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Acidic phospholipids have been shown to promote dissociation of bound nucleotides from Mycobacterium tuberculosis DnaA (DnaA(TB)) purified under denaturing conditions [Yamamoto et al., (2002) Modulation of Mycobacterium tuberculosis DnaA protein-adenine-nucleotide interactions by acidic phospholipids. Biochem. J., 363, 305-311]. In the present study, we show that a majority of DnaA(TB) in non-overproducing cells of M. tuberculosis is membrane associated. Estimation of phospholipid phosphorus following chloroform: methanol extraction of soluble DnaA(TB) purified under native conditions (nDnaA(TB)) confirmed the association with phospholipids. nDnaA(TB) exhibited weak ATPase activity, and rapidly exchanged ATP for bound ADP in the absence of any added phospholipids. We suggest that the outcome of intra-cellular DnaA(TB)-nucleotide interactions, hence DnaA(TB) activity, is influenced by phospholipids. |
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Authors:
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Kohji Yamamoto; Meredith Moomey; Malini Rajagopalan; Murty V V S Madiraju |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2008-02-22 |
Journal Detail:
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Title: Journal of biochemistry Volume: 143 ISSN: 0021-924X ISO Abbreviation: J. Biochem. Publication Date: 2008 Jun |
Date Detail:
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Created Date: 2008-06-27 Completed Date: 2008-09-30 Revised Date: 2011-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0376600 Medline TA: J Biochem Country: Japan |
Other Details:
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Languages: eng Pagination: 759-64 Citation Subset: IM |
Affiliation:
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Graduate School of Bioresource and Bioenvironmental Science, Kyushu University, Fukuoka 812-8581, Japan. yamamok@agr.kyushu-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphatases
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metabolism Bacterial Proteins / metabolism* Blotting, Western Cell Membrane / metabolism DNA Replication* DNA-Binding Proteins / metabolism* Mycobacterium tuberculosis / growth & development, metabolism* Nucleotides / metabolism* Phospholipids / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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AI41406/AI/NIAID NIH HHS; AI48417/AI/NIAID NIH HHS; R01 AI041406-07/AI/NIAID NIH HHS; R01 AI041406-08/AI/NIAID NIH HHS; R01 AI048417-07/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/DNA-Binding Proteins; 0/DnaA protein, Bacteria; 0/Nucleotides; 0/Phospholipids; EC 3.6.1.-/Adenosine Triphosphatases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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