| Facile analysis of short-chain fatty acids as 4-nitrophenyl esters in complex anaerobic fermentation samples by high performance liquid chromatography. | |
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MedLine Citation:
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PMID: 21782191 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Short-chain fatty acids are crucial intermediates in the conversion of biomass to methane. Due to the complexity of raw biomass, volatile fatty acids (including n- and branched-chain compounds) as well as arylacetic and arylpropionic acids arise from digestion of carbohydrates, proteins and lipids. The development of a simple extraction procedure in combination with internal standardization and facile 4-nitrophenyl-labelling via oxalylchloride-generated acylchlorides enabled robust separation and quantification of the target compounds in crude biological samples like raw cattle manure and biogas fermenter contents. Detection limits of <100μM and error rates of less than 4% for the quantification of individual compounds in a concentration range up to 50mM for non-diluted samples suggest that the novel method might be of general advantage for the routine quantification of short-chain fatty acids in complex biological samples including complex fermentation media. |
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Authors:
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Johannes Schiffels; Marcus E M Baumann; Thorsten Selmer |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-7-3 |
Journal Detail:
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Title: Journal of chromatography. A Volume: - ISSN: 1873-3778 ISO Abbreviation: - Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-7-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011. Published by Elsevier B.V. |
Affiliation:
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FH Aachen, Institut für Nano- und Biotechnologien, Heinrich-Mußmann-Str. 1, 52428 Jülich, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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