Document Detail


Fabrication of wool keratin sponge scaffolds for long-term cell cultivation.
MedLine Citation:
PMID:  11738723     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Wool keratin sponge scaffolds were fabricated by lyophilization of an aqueous wool keratin solution after controlled freezing. Freezing at -20 degrees C for 3 days was needed for the preparation of stable sponges, which did not show significant changes against heat treatment at 60 degrees C for several hours. Scanning electron microscopic observation revealed that the wool keratin sponges had a homogeneously porous microstructure, the pore size was approximately equal to 100 microm. At 1 h from seeding, the adhesion of cells was observed and at 1 day, cells spread on the sponge surface. Rapid cell growth on the sponge (doubling time: 29.0 h) was observed for at least 7 days, as well as on a commercially available plastic culture dish (doubling time: 27.4 h). At long-term (23-43 days) cultivation, cells were constantly counted to be approximately 4.2-7.4 million per sponge (1 cm in diameter). The maximum cell number was 7.4 million, approximately 37 times higher than on the same area dish. Living cells on the sponge were observed at 23-43 days by SEM observation and no abnormal morphology of the cells was observed. These results show that wool keratin sponges are useful scaffolds for long-term and high-density cell cultivation.
Authors:
Akira Tachibana; Yasunari Furuta; Hideyuki Takeshima; Toshizumi Tanabe; Kiyoshi Yamauchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biotechnology     Volume:  93     ISSN:  0168-1656     ISO Abbreviation:  J. Biotechnol.     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2001-12-12     Completed Date:  2002-02-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  165-70     Citation Subset:  IM    
Affiliation:
Department of Bioapplied Chemistry, Graduate School of Engineering, Osaka City University, Sugimoto 3-3-138, Sumiyoshi-ku, Osaka 558-8585, Japan. tatibana@bioa.eng.osaka-cu.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Culture Techniques
Cell Division
Cells / cytology*
Keratins*
Microscopy, Electron, Scanning
Wool*
Chemical
Reg. No./Substance:
68238-35-7/Keratins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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