| Fabrication of stratified nanoporous gold for enhanced biosensing. | |
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MedLine Citation:
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PMID: 22494542 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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By a dealloying/annealing/redealloying strategy, nanoporous gold (NPG) with hierarchical microstructure is fabricated for electrochemical biosensing application. The first dealloying and annealing would produce NPG/AuAg alloy composite with a large-pore NPG layer and the second dealloying would further etch the AuAg alloy part in the composite, generating a small-pore NPG layer. By using the large-pore (≈ 100 nm) layer as the glucose oxidase (GOx) container, and the small-pore (≈ 12 nm) layer as a signal producer, this novel hierarchical NPG is demonstrated to be a good support for enzyme immobilization and fabricating enzyme-based biosensors. The immobilized GOx retains ≈ 92% of the initial activity after 7 repeated use. The GOx-loaded stratified NPG biosensor can detect glucose more sensitively with a wider linear range (up to 22 mM) than normal NPG with a uniform pore size of 30-40 nm (linear range: up to 17 mM). |
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Authors:
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Huajun Qiu; Feixue Zou |
Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2012-03-18 |
Journal Detail:
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Title: Biosensors & bioelectronics Volume: 35 ISSN: 1873-4235 ISO Abbreviation: Biosens Bioelectron Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-05-08 Completed Date: 2012-09-26 Revised Date: 2012-11-07 |
Medline Journal Info:
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Nlm Unique ID: 9001289 Medline TA: Biosens Bioelectron Country: England |
Other Details:
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Languages: eng Pagination: 349-54 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Elsevier B.V. All rights reserved. |
Affiliation:
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School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China. qiuhuajun@gmail.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biosensing Techniques
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methods*,
statistics & numerical data Electrochemical Techniques Enzymes, Immobilized Glucose / analysis Glucose Oxidase Gold Metal Nanoparticles* / ultrastructure Microscopy, Electron, Scanning Nanotechnology Porosity |
| Chemical | |
Reg. No./Substance:
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0/Enzymes, Immobilized; 50-99-7/Glucose; 7440-57-5/Gold; EC 1.1.3.4/Glucose Oxidase |
| Comments/Corrections | |
Erratum In:
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Biosens Bioelectron. 2013 Jan 15;39(1):352-3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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