Document Detail

Fabrication of stratified nanoporous gold for enhanced biosensing.
MedLine Citation:
PMID:  22494542     Owner:  NLM     Status:  MEDLINE    
By a dealloying/annealing/redealloying strategy, nanoporous gold (NPG) with hierarchical microstructure is fabricated for electrochemical biosensing application. The first dealloying and annealing would produce NPG/AuAg alloy composite with a large-pore NPG layer and the second dealloying would further etch the AuAg alloy part in the composite, generating a small-pore NPG layer. By using the large-pore (≈ 100 nm) layer as the glucose oxidase (GOx) container, and the small-pore (≈ 12 nm) layer as a signal producer, this novel hierarchical NPG is demonstrated to be a good support for enzyme immobilization and fabricating enzyme-based biosensors. The immobilized GOx retains ≈ 92% of the initial activity after 7 repeated use. The GOx-loaded stratified NPG biosensor can detect glucose more sensitively with a wider linear range (up to 22 mM) than normal NPG with a uniform pore size of 30-40 nm (linear range: up to 17 mM).
Huajun Qiu; Feixue Zou
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-03-18
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  35     ISSN:  1873-4235     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-08     Completed Date:  2012-09-26     Revised Date:  2012-11-07    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  349-54     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China.
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MeSH Terms
Biosensing Techniques / methods*,  statistics & numerical data
Electrochemical Techniques
Enzymes, Immobilized
Glucose / analysis
Glucose Oxidase
Metal Nanoparticles* / ultrastructure
Microscopy, Electron, Scanning
Reg. No./Substance:
0/Enzymes, Immobilized; 50-99-7/Glucose; 7440-57-5/Gold; EC Oxidase
Erratum In:
Biosens Bioelectron. 2013 Jan 15;39(1):352-3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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