| Fabrication and release behavior of a novel freeze-gelled chitosan/gamma-PGA scaffold as a carrier for rhBMP-2. | |
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MedLine Citation:
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PMID: 16239024 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: The aim of this study was to fabricate a novel composite porous scaffold by blending chitosan and gamma-poly(glutamic acid) (gamma-PGA) for the sustained delivery of rhBMP-2. METHODS: Chitosan and gamma-PGA were blended to fabricate a novel porous scaffold by the freeze-gelation method. For comparison, scaffolds made of freeze-dried chitosan, freeze-dried PLLA, and freeze-gelled chitosan were also prepared. The scaffolds were loaded with rhBMP-2, and then the controlled release of rhBMP-2 from the scaffolds was assessed by ELISA. RESULTS: The freeze-gelled chitosan/gamma-PGA scaffold (M0=318.29 ng, k=0.32 d(-1)) gave the most satisfactory release curve, followed by the freeze-gelled chitosan (M0=392.76 ng, k=0.59 d(-1)), freeze-dried chitosan (M0=229.21 ng, k=2.28 d(-1)), and freeze-dried PLLA (M0=8.4 ng, k=482.54 d(-1)) scaffolds. In the stability test, p-dioxane (the solvent for PLLA) seriously deteriorated rhBMP-2, whereas acetic acid (the solvent for chitosan) did not. SIGNIFICANCE: A novel chitosan/gamma-PGA composite scaffold for the controlled release of rhBMP-2 was established, with an enhanced release amount and sustained release behavior. This scaffold has many potential applications in bone regenerative therapies. |
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Authors:
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Chieh-Yang Hsieh; Hsyue-Jen Hsieh; Hwa-Chuang Liu; Da-Ming Wang; Lein-Tuan Hou |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-10-18 |
Journal Detail:
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Title: Dental materials : official publication of the Academy of Dental Materials Volume: 22 ISSN: 0109-5641 ISO Abbreviation: Dent Mater Publication Date: 2006 Jul |
Date Detail:
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Created Date: 2006-06-13 Completed Date: 2006-08-29 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8508040 Medline TA: Dent Mater Country: England |
Other Details:
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Languages: eng Pagination: 622-9 Citation Subset: D |
Affiliation:
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Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan, ROC. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bone Morphogenetic Protein 2 Bone Morphogenetic Proteins / administration & dosage*, chemistry Chitosan* Delayed-Action Preparations Drug Carriers* Drug Stability Freeze Drying Gels Humans Microscopy, Electron, Scanning Polyglutamic Acid* Recombinant Proteins / administration & dosage Solvents Transforming Growth Factor beta / administration & dosage*, chemistry |
| Chemical | |
Reg. No./Substance:
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0/BMP2 protein, human; 0/Bone Morphogenetic Protein 2; 0/Bone Morphogenetic Proteins; 0/Delayed-Action Preparations; 0/Drug Carriers; 0/Gels; 0/Recombinant Proteins; 0/Solvents; 0/Transforming Growth Factor beta; 25513-46-6/Polyglutamic Acid; 9012-76-4/Chitosan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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