Document Detail


Fabrication of a multi-layer three-dimensional scaffold with controlled porous micro-architecture for application in small intestine tissue engineering.
MedLine Citation:
PMID:  23563499     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Various methods can be employed to fabricate scaffolds with characteristics that promote cell-to-material interaction. This report examines the use of a novel technique combining compression molding with particulate leaching to create a unique multi-layered scaffold with differential porosities and pore sizes that provides a high level of control to influence cell behavior. These cell behavioral responses were primarily characterized by bridging and penetration of two cell types (epithelial and smooth muscle cells) on the scaffold in vitro. Larger pore sizes corresponded to an increase in pore penetration, and a decrease in pore bridging. In addition, smaller cells (epithelial) penetrated further into the scaffold than larger cells (smooth muscle cells). In vivo evaluation of a multi-layered scaffold was well tolerated for 75 d in a rodent model. This data shows the ability of the components of multi-layered scaffolds to influence cell behavior, and demonstrates the potential for these scaffolds to promote desired tissue outcomes in vivo.
Authors:
Toyin Knight; Joydeep Basu; Elias A Rivera; Thomas Spencer; Deepak Jain; Richard Payne
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Publication Detail:
Type:  Journal Article     Date:  2013-04-05
Journal Detail:
Title:  Cell adhesion & migration     Volume:  7     ISSN:  1933-6926     ISO Abbreviation:  Cell Adh Migr     Publication Date:    2013 May-Jun
Date Detail:
Created Date:  2013-06-26     Completed Date:  2014-02-06     Revised Date:  2014-05-07    
Medline Journal Info:
Nlm Unique ID:  101469464     Medline TA:  Cell Adh Migr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  267-74     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Biocompatible Materials
Cell Line
Cell Movement
Epithelial Cells / metabolism
Intestine, Small*
Myocytes, Smooth Muscle / metabolism
Porosity
Rats
Surface Properties
Tissue Engineering / methods*
Tissue Scaffolds*
Chemical
Reg. No./Substance:
0/Biocompatible Materials
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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