| Fabin, a novel calcyon-like and glucanase-like protein with mitogenic, antifungal and translation-inhibitory activities from broad beans. | |
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MedLine Citation:
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PMID: 12817478 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A protein with an N-terminal sequence displaying similarities to N-terminal sequences of human calcyon and barley endo-1,4-glucanase, and to C-terminal sequences of human translation initiation factor 4 gamma and yeast superkiller viralicidic activity, was isolated from the broad bean Vicia faba. The protein, termed fabin, has a molecular mass of 34 kDa in SDS-polyacrylamide gel electrophoresis. Antifungal activity of the protein was observed against several fungal species including Rhizoctonia solani, Botrytis cinerea, Fusarium oxysporum and Mycosphaerella arachidicola. Fabin inhibits HIV-1 reverse transcriptase with an IC50 of 34 microM and translation in a rabbit reticulocyte lysate with an IC50 of 2.4 microM. At a concentration of about 1.5 microM fabin is able to elicit a 9-fold increase in the mitogenic response of murine splenocytes. |
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Authors:
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T B Ng; X Y Ye |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biological chemistry Volume: 384 ISSN: 1431-6730 ISO Abbreviation: Biol. Chem. Publication Date: 2003 May |
Date Detail:
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Created Date: 2003-06-23 Completed Date: 2004-02-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9700112 Medline TA: Biol Chem Country: Germany |
Other Details:
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Languages: eng Pagination: 811-5 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Faculty of Medicine, Chinese University of Hong Kong, Shatin, Hong Kong, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Antifungal Agents / isolation & purification, pharmacology* Cellulase / chemistry*, genetics, pharmacology Fungi / drug effects Humans Membrane Proteins / chemistry*, genetics, pharmacology Mice Mitogens / isolation & purification, pharmacology Molecular Weight Plant Proteins / genetics, isolation & purification, pharmacology* Protein Biosynthesis / drug effects Spleen / cytology Vicia faba / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Antifungal Agents; 0/Membrane Proteins; 0/Mitogens; 0/Plant Proteins; 0/calcyon; EC 3.2.1.4/Cellulase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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