Document Detail


Fabin, a novel calcyon-like and glucanase-like protein with mitogenic, antifungal and translation-inhibitory activities from broad beans.
MedLine Citation:
PMID:  12817478     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A protein with an N-terminal sequence displaying similarities to N-terminal sequences of human calcyon and barley endo-1,4-glucanase, and to C-terminal sequences of human translation initiation factor 4 gamma and yeast superkiller viralicidic activity, was isolated from the broad bean Vicia faba. The protein, termed fabin, has a molecular mass of 34 kDa in SDS-polyacrylamide gel electrophoresis. Antifungal activity of the protein was observed against several fungal species including Rhizoctonia solani, Botrytis cinerea, Fusarium oxysporum and Mycosphaerella arachidicola. Fabin inhibits HIV-1 reverse transcriptase with an IC50 of 34 microM and translation in a rabbit reticulocyte lysate with an IC50 of 2.4 microM. At a concentration of about 1.5 microM fabin is able to elicit a 9-fold increase in the mitogenic response of murine splenocytes.
Authors:
T B Ng; X Y Ye
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biological chemistry     Volume:  384     ISSN:  1431-6730     ISO Abbreviation:  Biol. Chem.     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-06-23     Completed Date:  2004-02-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9700112     Medline TA:  Biol Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  811-5     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Faculty of Medicine, Chinese University of Hong Kong, Shatin, Hong Kong, China.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antifungal Agents / isolation & purification,  pharmacology*
Cellulase / chemistry*,  genetics,  pharmacology
Fungi / drug effects
Humans
Membrane Proteins / chemistry*,  genetics,  pharmacology
Mice
Mitogens / isolation & purification,  pharmacology
Molecular Weight
Plant Proteins / genetics,  isolation & purification,  pharmacology*
Protein Biosynthesis / drug effects
Spleen / cytology
Vicia faba / chemistry*
Chemical
Reg. No./Substance:
0/Antifungal Agents; 0/Membrane Proteins; 0/Mitogens; 0/Plant Proteins; 0/calcyon; EC 3.2.1.4/Cellulase

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