| FTY720-loaded poly(DL-lactide-co-glycolide) Electrospun Scaffold Significantly Increases Microvessel Density over 7 Days in Streptozotocin-Induced Diabetic C57b16/J Mice: Preliminary Results. | |
| | |
MedLine Citation:
|
PMID: 22099778 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
BACKGROUND: Nanofiber scaffolds could improve islet transplant success by physically mimicking the shape of extracellular matrix and by acting as a drug-delivery vehicle. Scaffolds implanted in alternate transplant sites must be prevascularized or very quickly vascularized following transplantation to prevent hypoxia-induced islet necrosis. The local release of the S1P prodrug FTY720 induces diameter enlargement and increases in length density. The objective of this preliminary study was to evaluate length and diameter differences between diabetic and nondiabetic animals implanted with FTY720-containing electrospun scaffolds using intravital imaging of dorsal skinfold window chambers. METHODS: Electrospun mats of randomly oriented fibers we created from polymer solutions of PLAGA (50:50 LA:GA) with and without FTY720 loaded at a ratio of 1:200 (FTY720:PLAGA by wt). The implanted fiber mats were 4 mm in diameter and ∼0.2 mm thick. Increases in length density and vessel diameter were assessed by automated analysis of images over 7 days in RAVE, a Matlab program. RESULTS: Image analysis of repeated measures of microvessel metrics demonstrated a significant increase in the length density from day 0 to day 7 in the moderately diabetic animals of this preliminary study (P < .05). Furthermore, significant differences in length density at day 0 and day 3 were found between recently STZ-induced moderately diabetic and nondiabetic animals in response to FTY720 local release (P < .05, Student t test). CONCLUSIONS: Driving the islet revascularization process using local release of factors, such as FTY720, from biodegradable polymers makes an attractive system for the improvement of islet transplant success. Preliminary study results suggest that a recently induced moderately diabetic state may potentiate the mechanism by which local release of FTY720 from polymer fibers increases length density of microvessels. Therefore, local release of S1P receptor-targeted drugs is under further investigation for improvement of transplanted islet function. |
| | |
Authors:
|
D T Bowers; P Chhabra; L Langman; E A Botchwey; K L Brayman |
Related Documents
:
|
16344038 - Management of diabetes during acute stroke and inpatient stroke rehabilitation. 22265768 - The outcome of manipulation under general anesthesia for the management of frozen shoul... 16280338 - Diabetes and schizophrenia 2005: are we any closer to understanding the link? 21680718 - Cluster analysis of self-monitoring blood glucose assessments in clinical islet cell tr... 14976468 - Role of oral anti-diabetic agents in modifying cardiovascular risk factors. 4008898 - Gluconeogenesis by the fetal sheep liver in vivo. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Transplantation proceedings Volume: 43 ISSN: 1873-2623 ISO Abbreviation: Transplant. Proc. Publication Date: 2011 Nov |
Date Detail:
|
Created Date: 2011-11-21 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0243532 Medline TA: Transplant Proc Country: United States |
Other Details:
|
Languages: eng Pagination: 3285-7 Citation Subset: IM |
Copyright Information:
|
Copyright © 2011. Published by Elsevier Inc. |
Affiliation:
|
Biomedical Engineering, University of Virginia, Charlottesville, Virginia. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Assessment of Mitochondrial DNA as an Indicator of Islet Quality: An Example in Goto Kakizaki Rats.
Next Document: Neoplasm incidence in simultaneous pancreas and kidney transplantation: a single-center analysis.