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The FTIR spectra of algal species can be used as physiological fingerprints to assess their actual growth potential.
MedLine Citation:
PMID:  22540209     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Fourier transform infrared (FTIR) spectra were measured from cells of Microcystis aeruginosa and Protoceratium reticulatum, whose growth rates were manipulated by the availability of nutrients or light. As expected, the macromolecular composition changed in response to the treatments. These changes were species-specific and depended on the type of perturbation applied to the growth regime. M. aeruginosa showed an increase in the carbohydrate to protein ratio with decreased growth rates, under nutrient limitation, whereas light limitation induced a decrease of the carbohydrate to protein ratio with decreasing proliferation rates. The macromolecular pools of P. reticulatum showed a higher degree of compositional homeostasis. Only when the lowest light irradiance and nutrient availability were supplied an increase of the carbohydrate to protein FTIR absorbance ratio was observed. A species-specific partial least square (PLS) model was developed using the whole FTIR spectra. This model afforded a very high correlation between the predicted and the measured growth rates, regardless of the growth conditions. On the contrary, the prediction based on absorption band ratios usually used in FTIR studies would strongly depend on growth conditions. This new computational method could constitute a substantial improvement in the early warning systems of algal blooms and in general for the study of algal growth, e.g. in biotechnology. Furthermore, these results confirm the suitability of FTIR spectroscopy as a tool to map complex biological processes like growth under different environmental conditions.
Authors:
Christian Jebsen; Alessandra Norici; Heiko Wagner; Matteo Palmucci; Mario Giordano; Christian Wilhelm
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-27
Journal Detail:
Title:  Physiologia plantarum     Volume:  -     ISSN:  1399-3054     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1256322     Medline TA:  Physiol Plant     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © Physiologia Plantarum 2012.
Affiliation:
Institut of Biology - Plant Physiology, Universität Leipzig, Johannisallee 23, D-04103 Leipzig, Germany Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.
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