Document Detail

FT-Raman spectroscopy as diagnostic tool of Congo red binding to amyloids.
MedLine Citation:
PMID:  12722114     Owner:  NLM     Status:  MEDLINE    
Chorion is the major component of silkmoth eggshell. More than 95% of its dry mass consists of the A and B families of low molecular weight structural proteins, which have remarkable mechanical and chemical properties protecting the oocyte and developing embryo from environmental hazards. We present data from FT-Raman spectroscopy of silkmoth chorion and amyloid-like fibrils formed from peptide analogues of chorion proteins, both unstained and stained by Congo red. The results show that FT-Raman spectroscopy is not a straightforward diagnostic tool for the specific interactions of Congo red with amyloids: a dilute aqueous solution of the Congo red dye at pH 5.5 and a thin solid film of the dye cast from this solution exhibit the same "diagnostic" Raman shifts relative to the neat Congo red dry powder as do amyloid fibrils formed from peptide analogues of chorion proteins stained by Congo red. An important consequence of this finding is that these shifts of the Raman active modes of Congo red are probably due to the formation of supramolecular dye aggregates in the presence of water. Therefore, this is not an appropriate diagnostic test for Congo red binding to amyloids.
Vassiliki A Iconomidou; Georgios D Chryssikos; Vassilis Gionis; Andreas Hoenger; Stavros J Hamodrakas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biopolymers     Volume:  72     ISSN:  0006-3525     ISO Abbreviation:  Biopolymers     Publication Date:  2003  
Date Detail:
Created Date:  2003-04-30     Completed Date:  2003-06-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372525     Medline TA:  Biopolymers     Country:  United States    
Other Details:
Languages:  eng     Pagination:  185-92     Citation Subset:  IM    
Copyright Information:
Copyright 2003 Wiley Periodicals, Inc.
Department of Cell Biology and Biophysics, University of Athens, Panepistimiopolis, Athens 157 01, Greece.
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MeSH Terms
Bombyx / metabolism
Congo Red / metabolism*
Egg Proteins / metabolism*,  ultrastructure
Microscopy, Electron
Spectrum Analysis, Raman*
Staining and Labeling / methods*
Reg. No./Substance:
0/Egg Proteins; 0/chorion proteins; 573-58-0/Congo Red

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