| An FRET-based ratiometric chemosensor for in vitro cellular fluorescence analyses of pH. | |
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MedLine Citation:
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PMID: 21982292 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ratiometric fluorescence sensing is an important technique for precise and quantitative analysis of biological events occurring under complex conditions by simultaneously recording fluorescence intensities at two wavelengths and calculating their ratios. Herein, we design a ratiometric chemosensor for pH that is based on photo-induced electron transfer (PET) and binding-induced modulation of fluorescence resonance energy transfer (FRET) mechanisms. This ratiometric chemosensor was constructed by introduction of a pH-insensitive coumarin fluorophore as an FRET donor into a pH-sensitive amino-naphthalimide derivative as the FRET acceptor. The sensor exhibited clear dual-mission signal changes in blue and green spectral windows upon pH changes. The pH sensor was applied for not only measuring cellular pH, but also for visualizing stimulus-responsive changes of intracellular pH values. |
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Authors:
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Xianfeng Zhou; Fengyu Su; Hongguang Lu; Patti Senechal-Willis; Yanqing Tian; Roger H Johnson; Deirdre R Meldrum |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-10-07 |
Journal Detail:
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Title: Biomaterials Volume: 33 ISSN: 1878-5905 ISO Abbreviation: Biomaterials Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2011-11-04 Completed Date: 2012-05-23 Revised Date: 2013-02-20 |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 171-80 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Ltd. All rights reserved. |
Affiliation:
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Center for Biosignatures Discovery Automation, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biosensing Techniques / methods* Cell Line Diagnostic Imaging / methods* Fluorescence Resonance Energy Transfer / methods* HeLa Cells Humans Hydrogen-Ion Concentration Magnetic Resonance Spectroscopy Mice Spectrometry, Fluorescence |
| Grant Support | |
ID/Acronym/Agency:
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5P50 HG002360/HG/NHGRI NIH HHS; P50 HG002360-09S1/HG/NHGRI NIH HHS; P50 HG002360-10/HG/NHGRI NIH HHS; P50 HG002360-10S1/HG/NHGRI NIH HHS; P50 HG002360-11/HG/NHGRI NIH HHS |
| Comments/Corrections | |
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