Document Detail

FRET and FRAP imaging: approaches to characterize tau and stathmin interactions with microtubules in cells.
MedLine Citation:
PMID:  23312015     Owner:  NLM     Status:  Publisher    
Microtubules (MTs) are involved in many crucial processes such as cell morphogenesis, mitosis and motility. These dynamic structures resulting from the complex assembly of tubulin are tightly regulated by stabilizing microtubule-associated proteins (MAPs) such as tau and destabilizing proteins, notably stathmin. Because of their key role, these MAPs and their interactions have been extensively studied using biochemical and biophysical approaches, particularly in vitro. Nevertheless, numerous questions remain unanswered and the mechanisms of interaction between MT and these proteins are still unclear in cells. Techniques coupling cell imaging and fluorescence methods, such as FRET and FRAP, are excellent tools to study these interactions in situ. After describing these methods, we will present emblematic data from the literature and unpublished experimental results from our laboratory concerning the interactions between microtubules, tau and stathmin in cells.
Roqiya Nouar; François Devred; Gilles Breuzard; Vincent Peyrot
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-14
Journal Detail:
Title:  Biology of the cell / under the auspices of the European Cell Biology Organization     Volume:  -     ISSN:  1768-322X     ISO Abbreviation:  Biol. Cell     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8108529     Medline TA:  Biol Cell     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 Soçiété Francaise des Microscopies and Société de Biologie Cellulaire de France.
INSERM UMR 911, Aix-Marseille Université, CRO2, 13385, Marseille, France.
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