Document Detail

FOXP3, CBLB and ITCH gene expression and cytotoxic T lymphocyte antigen 4 expression on CD4(+) CD25(high) T cells in multiple sclerosis.
MedLine Citation:
PMID:  23039885     Owner:  NLM     Status:  MEDLINE    
Expression of the forkhead box protein 3 (FoxP3) transcription factor is regulated by the E3 ubiquitin ligases Itch and Cbl-b and induces regulatory activity CD4(+) CD25(high) T cells. Treatment with interferon (IFN)-β enhances regulatory T cell activity in multiple sclerosis (MS). We studied the phenotype of CD4(+) CD25(high) T cells in MS by flow cytometry and its relationship with expression of the FOXP3, ITCH and CBLB genes. We found that untreated MS patients had lower cell surface expression of cytotoxic T lymphocyte antigen 4 (CTLA-4) on CD4(+) CD25(high) T cells and higher intracellular CTLA-4 expression than healthy controls. Cell surface expression of CTLA-4 on CD4(+) CD25(high) T cells correlated with expression of FOXP3 mRNA in untreated patients and increased significantly with time from most recent injection in patients treated with IFN-β. FOXP3 mRNA expression correlated with CBLB and ITCH and T helper type 2 cytokine mRNA expression in MS patients. These data link expression of FOXP3, CBLB and ITCH mRNA and CTLA-4 expression on the surface of CD4(+) CD25(high) T cell in MS. We hypothesize that this may reflect alterations in the inhibitory effect of CTLA-4 or in regulatory T cell function.
F Sellebjerg; M Krakauer; M Khademi; T Olsson; P S Sørensen
Related Documents :
22927405 - Pathogenic bacterium helicobacter pylori alters the expression profile of p53 protein i...
24631505 - Reversible acetylation of lin28 mediated by pcaf and sirt1.
24386415 - A novel role for an ecf sigma factor in fatty acid biosynthesis and membrane fluidity i...
24318885 - Directing rnase p-mediated cleavage of target mrnas by engineered external guide sequen...
25092915 - Metastasis-associated protein ribosomal rna processing 1 homolog b (rrp1b) modulates me...
20213765 - Preservation of the chondrocyte's pericellular matrix improves cell-induced cartilage f...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  170     ISSN:  1365-2249     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-08     Completed Date:  2013-03-18     Revised Date:  2013-11-05    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  149-55     Citation Subset:  IM    
Copyright Information:
© 2012 The Authors Clinical and Experimental Immunology © 2012 British Society for Immunology.
Danish Multiple Sclerosis Center, Department of Neurology, Copenhagen University Hospital Rigshospitalet, Copenhagen, Denmark.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Adaptor Proteins, Signal Transducing / biosynthesis,  genetics*,  immunology
CD4-Positive T-Lymphocytes / immunology*
CTLA-4 Antigen / biosynthesis,  genetics*,  immunology
Forkhead Transcription Factors / biosynthesis,  genetics*,  immunology
Gene Expression / immunology
Interferon-beta / biosynthesis,  genetics,  immunology
Interleukin-2 Receptor alpha Subunit / biosynthesis,  genetics*,  immunology
Multiple Sclerosis / genetics*,  immunology
Proto-Oncogene Proteins c-cbl / biosynthesis,  genetics*,  immunology
RNA, Messenger / genetics,  immunology
Repressor Proteins / biosynthesis,  genetics*,  immunology
Ubiquitin-Protein Ligases / biosynthesis,  genetics*,  immunology
Reg. No./Substance:
0/Adaptor Proteins, Signal Transducing; 0/CTLA-4 Antigen; 0/CTLA4 protein, human; 0/FOXP3 protein, human; 0/Forkhead Transcription Factors; 0/IL2RA protein, human; 0/Interleukin-2 Receptor alpha Subunit; 0/RNA, Messenger; 0/Repressor Proteins; 77238-31-4/Interferon-beta; EC 6.3.2.-/Proto-Oncogene Proteins c-cbl; EC protein, human; EC protein, human; EC Ligases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Antibody combination therapy targeting CD25, CD70 and CD8 reduces islet inflammation and improves gl...
Next Document:  Treatment with Fms-like tyrosine kinase 3 ligand reverses lung dendritic cell immunoparalysis and am...