Document Detail

FOXO3a is involved in the apoptosis of naked oocytes and oocytes of primordial follicles from neonatal rat ovaries.
MedLine Citation:
PMID:  19258007     Owner:  NLM     Status:  MEDLINE    
Inhibition of the forkhead transcription factor, FOXO3a, can promote the transition from primordial to primary follicle and subsequent follicle development in mammalian ovaries. Stem cell factor (SCF) initiates anti-apoptotic signaling from its membrane receptor, c-kit, to Bcl-2 family members through PI3K/AKT in oocytes of primordial follicles. However, whether FOXO3a mediates the apoptosis of naked oocytes and oocytes of primordial follicles remains unknown. In the present study, oocytes from nests and primordial follicles from neonatal rat ovaries were cultured, and oocyte apoptosis was examined using the TUNEL technique. The pro-apoptotic action of FOXO3a and the potential signal transduction pathways were investigated using RT-PCR, Western blot, and immunocytochemistry. Culturing oocytes in the presence of SCF did not affect the level of total FOXO3a protein, but rapidly elevated the level of phosphorylated FOXO3a (indicating functional suppression). As phosphorylated FOXO3a increased, oocyte apoptosis was inhibited. The specific PI3K/Akt inhibitor, LY 294002, abolished the phosphorylation of FOXO3a and the anti-apoptotic action of SCF. SCF down-regulated the expression of p27KIP1 and pro-apoptotic factors such as Bim, Bad, and Bax, and this activity was reversed by LY 294002. SCF up-regulated the expression of MnSOD, which was also inhibited by LY 294002. However, SCF had no effect on Bcl-2 protein. These results suggest that FOXO3a is involved in oocyte apoptosis in the neonatal rat ovary, and the SCF-PI3K/Akt-FOXO3a signaling pathway mediates oocyte apoptosis and primordial follicle formation.
Hong Liu; Li-Li Luo; Yuan-Shu Qian; Yu-Cai Fu; Xu-Xia Sui; Yi-Jie Geng; Da-Na Huang; Shi-Tong Gao; Ren-Li Zhang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-01
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  381     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-03-30     Completed Date:  2009-04-28     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  722-7     Citation Subset:  IM    
Shantou University Medical College, Shantou, Guangdong, PR China.
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MeSH Terms
Cyclin-Dependent Kinase Inhibitor p27 / metabolism
Forkhead Transcription Factors / genetics,  metabolism*
Oocytes / metabolism,  physiology*
Ovarian Follicle / metabolism,  physiology*
Proto-Oncogene Proteins c-akt / metabolism
Proto-Oncogene Proteins c-bcl-2 / metabolism
Rats, Sprague-Dawley
Stem Cell Factor / metabolism
Superoxide Dismutase / metabolism
Reg. No./Substance:
0/Forkhead Transcription Factors; 0/Foxo3a protein, rat; 0/Proto-Oncogene Proteins c-bcl-2; 0/Stem Cell Factor; 147604-94-2/Cyclin-Dependent Kinase Inhibitor p27; EC Dismutase; EC Proteins c-akt

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